Ultrathin-layer gel electrophoresis of biopolymers

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Emerging need for large-scale, high-resolution analysis of biopolymers, such as DNA sequencing polymerase chain reaction, (PCR) product sizing, single nucleotide polymorphism (SNP) hunting and analysis of protein molecules necessitated the development of automated and high-throughput gel electrophoresis based methods enabling rapid, high-performance separations in a wide molecular weight range. Scaling down electric field mediated separation processes supports higher throughput due to the applicability of higher voltages, thus speeding up analysis time. Indeed, efforts in miniaturization resulted in faster, easier, less costly and more convenient analyses, fulfilling the needs of the emerging biotechnology industry for microscale and massively parallel assays. The two primary approaches in miniaturizing electrophoresis dimensions are the capillary and microslab formats. This latter one evolved towards ultrathin-layer gel electrophoresis which is, except from the thickness of the separation platform, slightly in the upper side of the scale, resulting in considerably easier handling. Ultrathin-layer gel electrophoresis combines the advantages of conventional slab-gel electrophoresis (multilane format) and capillary gel electrophoresis (rapid, high-efficiency separations). It is readily automated, automatic versions of it have been extensively used for large-scale DNA sequencing in the Human Genome Project and more recently became popular in high throughput DNA fragment analysis. Ultrathin-layer techniques are the first step towards the wider use of electrophoresis microchips in perfecting a user-friendly interface between the user and the microdevice.

Original languageEnglish
Pages (from-to)3952-3964
Number of pages13
JournalElectrophoresis
Volume21
Issue number18
DOIs
Publication statusPublished - 2000

Fingerprint

Biopolymers
Electrophoresis
Gels
Capillary Electrophoresis
DNA Sequence Analysis
Microchip Electrophoresis
Throughput
Human Genome Project
Miniaturization
DNA-Directed DNA Polymerase
Biotechnology
Single Nucleotide Polymorphism
Industry
Polymerase chain reaction
DNA
Molecular Weight
Polymorphism
Reaction products
User interfaces
Polymerase Chain Reaction

Keywords

  • DNA
  • High-throughput separation
  • Microelectrophoresis
  • Microgel
  • Protein
  • Review
  • Ultrathin-layer gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Ultrathin-layer gel electrophoresis of biopolymers. / Guttman, A.; Rónai, Z.

In: Electrophoresis, Vol. 21, No. 18, 2000, p. 3952-3964.

Research output: Contribution to journalArticle

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