Ultrastructure of gametes after intracytoplasmic sperm injection

C. Bourgain, P. Nagy, H. De Zutter, H. Van Ranst, D. Nogueira, A. C. Van Steirteghem

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The ultrastructure of 18 metaphase-II oocytes after intracytoplasmic sperm injection (ICSI) was analysed from 30 min to 8 h following the microinjection. Three control metaphase-II oocytes were not injected. The 21 oocytes were embedded individually and examined in serial pole-to-pole sections. Seventeen oocytes had been matured in vitro from the germinal vesicle stage and showed ultrastructural alterations due to long-term culture. In microinjected oocytes, membrane-bound vacuoles and oolemma inclusions were found at the injection site. The ICSI oocytes showed evidence of plasma-membrane damage and increased oocyte exchange processes with residual and multivesiculated bodies. Gamete membrane fusion was not observed, Acrosome reaction was observed within the ooplasm 15 min after ICSI. Sperm elements were not incorporated into any oocyte vacuole, Onset of oocyte activation was associated with sperm chromatin decondensation. Cortical granules were released at 60 min and two pronuclei were formed at 6 h after ICSI. The male and female pronucleus formation was asynchronous, The findings suggest specific ICSI-related morphological features of early fertilization.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalHuman Reproduction
Volume13
Issue numberSUPPL. 1
Publication statusPublished - Apr 1998

Fingerprint

Intracytoplasmic Sperm Injections
Germ Cells
Oocytes
Metaphase
Vacuoles
Spermatozoa
Acrosome Reaction
Membrane Fusion
Microinjections
Fertilization
Chromatin
Cell Membrane
Injections
Membranes

Keywords

  • Intracytoplasmic sperm injection
  • Oocytes
  • Spermatozoa
  • Ultrastructure

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Bourgain, C., Nagy, P., De Zutter, H., Van Ranst, H., Nogueira, D., & Van Steirteghem, A. C. (1998). Ultrastructure of gametes after intracytoplasmic sperm injection. Human Reproduction, 13(SUPPL. 1), 107-116.

Ultrastructure of gametes after intracytoplasmic sperm injection. / Bourgain, C.; Nagy, P.; De Zutter, H.; Van Ranst, H.; Nogueira, D.; Van Steirteghem, A. C.

In: Human Reproduction, Vol. 13, No. SUPPL. 1, 04.1998, p. 107-116.

Research output: Contribution to journalArticle

Bourgain, C, Nagy, P, De Zutter, H, Van Ranst, H, Nogueira, D & Van Steirteghem, AC 1998, 'Ultrastructure of gametes after intracytoplasmic sperm injection', Human Reproduction, vol. 13, no. SUPPL. 1, pp. 107-116.
Bourgain C, Nagy P, De Zutter H, Van Ranst H, Nogueira D, Van Steirteghem AC. Ultrastructure of gametes after intracytoplasmic sperm injection. Human Reproduction. 1998 Apr;13(SUPPL. 1):107-116.
Bourgain, C. ; Nagy, P. ; De Zutter, H. ; Van Ranst, H. ; Nogueira, D. ; Van Steirteghem, A. C. / Ultrastructure of gametes after intracytoplasmic sperm injection. In: Human Reproduction. 1998 ; Vol. 13, No. SUPPL. 1. pp. 107-116.
@article{3a7a8d3fc942400eb1905ae33f299cec,
title = "Ultrastructure of gametes after intracytoplasmic sperm injection",
abstract = "The ultrastructure of 18 metaphase-II oocytes after intracytoplasmic sperm injection (ICSI) was analysed from 30 min to 8 h following the microinjection. Three control metaphase-II oocytes were not injected. The 21 oocytes were embedded individually and examined in serial pole-to-pole sections. Seventeen oocytes had been matured in vitro from the germinal vesicle stage and showed ultrastructural alterations due to long-term culture. In microinjected oocytes, membrane-bound vacuoles and oolemma inclusions were found at the injection site. The ICSI oocytes showed evidence of plasma-membrane damage and increased oocyte exchange processes with residual and multivesiculated bodies. Gamete membrane fusion was not observed, Acrosome reaction was observed within the ooplasm 15 min after ICSI. Sperm elements were not incorporated into any oocyte vacuole, Onset of oocyte activation was associated with sperm chromatin decondensation. Cortical granules were released at 60 min and two pronuclei were formed at 6 h after ICSI. The male and female pronucleus formation was asynchronous, The findings suggest specific ICSI-related morphological features of early fertilization.",
keywords = "Intracytoplasmic sperm injection, Oocytes, Spermatozoa, Ultrastructure",
author = "C. Bourgain and P. Nagy and {De Zutter}, H. and {Van Ranst}, H. and D. Nogueira and {Van Steirteghem}, {A. C.}",
year = "1998",
month = "4",
language = "English",
volume = "13",
pages = "107--116",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Ultrastructure of gametes after intracytoplasmic sperm injection

AU - Bourgain, C.

AU - Nagy, P.

AU - De Zutter, H.

AU - Van Ranst, H.

AU - Nogueira, D.

AU - Van Steirteghem, A. C.

PY - 1998/4

Y1 - 1998/4

N2 - The ultrastructure of 18 metaphase-II oocytes after intracytoplasmic sperm injection (ICSI) was analysed from 30 min to 8 h following the microinjection. Three control metaphase-II oocytes were not injected. The 21 oocytes were embedded individually and examined in serial pole-to-pole sections. Seventeen oocytes had been matured in vitro from the germinal vesicle stage and showed ultrastructural alterations due to long-term culture. In microinjected oocytes, membrane-bound vacuoles and oolemma inclusions were found at the injection site. The ICSI oocytes showed evidence of plasma-membrane damage and increased oocyte exchange processes with residual and multivesiculated bodies. Gamete membrane fusion was not observed, Acrosome reaction was observed within the ooplasm 15 min after ICSI. Sperm elements were not incorporated into any oocyte vacuole, Onset of oocyte activation was associated with sperm chromatin decondensation. Cortical granules were released at 60 min and two pronuclei were formed at 6 h after ICSI. The male and female pronucleus formation was asynchronous, The findings suggest specific ICSI-related morphological features of early fertilization.

AB - The ultrastructure of 18 metaphase-II oocytes after intracytoplasmic sperm injection (ICSI) was analysed from 30 min to 8 h following the microinjection. Three control metaphase-II oocytes were not injected. The 21 oocytes were embedded individually and examined in serial pole-to-pole sections. Seventeen oocytes had been matured in vitro from the germinal vesicle stage and showed ultrastructural alterations due to long-term culture. In microinjected oocytes, membrane-bound vacuoles and oolemma inclusions were found at the injection site. The ICSI oocytes showed evidence of plasma-membrane damage and increased oocyte exchange processes with residual and multivesiculated bodies. Gamete membrane fusion was not observed, Acrosome reaction was observed within the ooplasm 15 min after ICSI. Sperm elements were not incorporated into any oocyte vacuole, Onset of oocyte activation was associated with sperm chromatin decondensation. Cortical granules were released at 60 min and two pronuclei were formed at 6 h after ICSI. The male and female pronucleus formation was asynchronous, The findings suggest specific ICSI-related morphological features of early fertilization.

KW - Intracytoplasmic sperm injection

KW - Oocytes

KW - Spermatozoa

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0031833077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031833077&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 107

EP - 116

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - SUPPL. 1

ER -