Ultrastructure and immunoelectron microscopy of human T-cell leukaemia virus type I-producing lymphoid and non-lymphoid human tumour cells

J. Tímár, K. Nagy, D. Robertson, P. Clapham, R. A. Weiss

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The ultrastructure and distribution of some viral and cellular antigens was examined in lymphoid C91/PL and fibroblastic HOS/PL cells infected with human T-cell leukaemia virus type I. Tubuloreticular structures in the endoplasmic reticulum, characteristic of virus-infected or interferon-treated cells, were found in both cell types. Virions were observed particularly in the HOS/PL cells in budding and mature forms and in coated pits, coated vesicles and lysosomes, indicative of receptor-mediated endocytosis. Using immunogold indirect labelling, viral antigens, recognized by sera of infected patients and by monoclonal antibody, were detected in patches on the cell surface and in material loosely attached to the envelope of some virions. Beta2-microglobulin was associated with virion envelopes from C91/PL and HOS/PL cells, but major histocompatibility complex class I (HLA) antigens were not associated with virions even when produced by HLA-positive HOS/PL cells.

Original languageEnglish
Pages (from-to)1011-1020
Number of pages10
JournalJournal of General Virology
Volume68
Issue number4
Publication statusPublished - 1987

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Human T-lymphotropic virus 1
Immunoelectron Microscopy
Virion
Neoplasms
Viral Antigens
Coated Vesicles
Histocompatibility Antigens Class I
HLA Antigens
Endocytosis
Lysosomes
Major Histocompatibility Complex
Endoplasmic Reticulum
Interferons
Monoclonal Antibodies
Viruses
Serum

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Ultrastructure and immunoelectron microscopy of human T-cell leukaemia virus type I-producing lymphoid and non-lymphoid human tumour cells. / Tímár, J.; Nagy, K.; Robertson, D.; Clapham, P.; Weiss, R. A.

In: Journal of General Virology, Vol. 68, No. 4, 1987, p. 1011-1020.

Research output: Contribution to journalArticle

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