Ultrastructural pathology of degenerating "dark" granule cells in the hippocampal dentate gyrus of adrenalectomized rats

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Abstract

Adrenalectomy-evoked delayed degeneration and death of granule cells in the hippocampal dentate gyrus (DG) of the rat brain were studied by means of electron microscopy and a recently elaborated silver method that selectively stains the "dark", collapsed neurons in a Golgi-like manner. At the light microscopic level, the silver technique revealed degenerating granule cells located exclusively in the dentate gyrus; other glucocorticoid receptor-containing regions of the brain were not affected. The silver-stained cell bodies were shrunken, most of the dendrites had a beaded appearance, and the stained axons could be traced along their route to the CA3 pyramidal neurons of the hippocampus. The analysis of 2.5 μm thick Epon-embedded sections stained with toluidine blue revealed hyperchromatic, dark granule neurons and their remains and a heavy glial activity in the vicinity of collapsing neuronal profiles. At the ultrastructural level, early and late stages of neuronal degeneration were observed. The early phase was characterized by markedly increased electron density, a massive shrinkage of the whole somato-dendritic domain, vacuolization of mitochondria, swelling of the nucleolus and condensation of the nuclear chromatin. In the late stage, subcellular organelles were hardly recognizable due to the extremely high electron density and dramatic shrinkage of the cytoplasm. These profiles exhibited disintegration of the cellular organelles and loss of their afferents. Concomitantly, disintegration of granule cell dendrites (clasmatodendrosis) and lifting of "dark" mossy fibers from cell bodies and dendrites of CA3 pyramidal neurons were observed. In the latter cells, this partial denervation caused no apparent signs of ultrastructural alterations. Proliferation of astrocytes and microglial cells was also obvious as they engulfed and eliminated the degenerating neuronal elements. Degenerating neurons frequently occurred adjacent neurons with normal morphology. These morphological features indicate that the delayed degeneration of hippocampal granule cells following adrenalectomy might proceed through a cytoskeletal collapse terminating in cell death.

Original languageEnglish
Pages (from-to)173-187
Number of pages15
JournalActa Biologica Hungarica
Volume48
Issue number2
Publication statusPublished - 1997

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Parahippocampal Gyrus
Dentate Gyrus
Pathology
pathology
Neurons
Rats
granules
silver
electron density
Dendrites
Silver
brain
neurons
rats
Pyramidal Cells
Adrenalectomy
dendrites
Disintegration
Organelles
cells

Keywords

  • Adrenalectomy
  • Dark neurons
  • Dentate gyrus
  • Electron microscopy
  • Silver intensification

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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title = "Ultrastructural pathology of degenerating {"}dark{"} granule cells in the hippocampal dentate gyrus of adrenalectomized rats",
abstract = "Adrenalectomy-evoked delayed degeneration and death of granule cells in the hippocampal dentate gyrus (DG) of the rat brain were studied by means of electron microscopy and a recently elaborated silver method that selectively stains the {"}dark{"}, collapsed neurons in a Golgi-like manner. At the light microscopic level, the silver technique revealed degenerating granule cells located exclusively in the dentate gyrus; other glucocorticoid receptor-containing regions of the brain were not affected. The silver-stained cell bodies were shrunken, most of the dendrites had a beaded appearance, and the stained axons could be traced along their route to the CA3 pyramidal neurons of the hippocampus. The analysis of 2.5 μm thick Epon-embedded sections stained with toluidine blue revealed hyperchromatic, dark granule neurons and their remains and a heavy glial activity in the vicinity of collapsing neuronal profiles. At the ultrastructural level, early and late stages of neuronal degeneration were observed. The early phase was characterized by markedly increased electron density, a massive shrinkage of the whole somato-dendritic domain, vacuolization of mitochondria, swelling of the nucleolus and condensation of the nuclear chromatin. In the late stage, subcellular organelles were hardly recognizable due to the extremely high electron density and dramatic shrinkage of the cytoplasm. These profiles exhibited disintegration of the cellular organelles and loss of their afferents. Concomitantly, disintegration of granule cell dendrites (clasmatodendrosis) and lifting of {"}dark{"} mossy fibers from cell bodies and dendrites of CA3 pyramidal neurons were observed. In the latter cells, this partial denervation caused no apparent signs of ultrastructural alterations. Proliferation of astrocytes and microglial cells was also obvious as they engulfed and eliminated the degenerating neuronal elements. Degenerating neurons frequently occurred adjacent neurons with normal morphology. These morphological features indicate that the delayed degeneration of hippocampal granule cells following adrenalectomy might proceed through a cytoskeletal collapse terminating in cell death.",
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T1 - Ultrastructural pathology of degenerating "dark" granule cells in the hippocampal dentate gyrus of adrenalectomized rats

AU - Liposits, Z.

AU - Kalló, I.

AU - Hrabovszky, E.

AU - Gallyas, F.

PY - 1997

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N2 - Adrenalectomy-evoked delayed degeneration and death of granule cells in the hippocampal dentate gyrus (DG) of the rat brain were studied by means of electron microscopy and a recently elaborated silver method that selectively stains the "dark", collapsed neurons in a Golgi-like manner. At the light microscopic level, the silver technique revealed degenerating granule cells located exclusively in the dentate gyrus; other glucocorticoid receptor-containing regions of the brain were not affected. The silver-stained cell bodies were shrunken, most of the dendrites had a beaded appearance, and the stained axons could be traced along their route to the CA3 pyramidal neurons of the hippocampus. The analysis of 2.5 μm thick Epon-embedded sections stained with toluidine blue revealed hyperchromatic, dark granule neurons and their remains and a heavy glial activity in the vicinity of collapsing neuronal profiles. At the ultrastructural level, early and late stages of neuronal degeneration were observed. The early phase was characterized by markedly increased electron density, a massive shrinkage of the whole somato-dendritic domain, vacuolization of mitochondria, swelling of the nucleolus and condensation of the nuclear chromatin. In the late stage, subcellular organelles were hardly recognizable due to the extremely high electron density and dramatic shrinkage of the cytoplasm. These profiles exhibited disintegration of the cellular organelles and loss of their afferents. Concomitantly, disintegration of granule cell dendrites (clasmatodendrosis) and lifting of "dark" mossy fibers from cell bodies and dendrites of CA3 pyramidal neurons were observed. In the latter cells, this partial denervation caused no apparent signs of ultrastructural alterations. Proliferation of astrocytes and microglial cells was also obvious as they engulfed and eliminated the degenerating neuronal elements. Degenerating neurons frequently occurred adjacent neurons with normal morphology. These morphological features indicate that the delayed degeneration of hippocampal granule cells following adrenalectomy might proceed through a cytoskeletal collapse terminating in cell death.

AB - Adrenalectomy-evoked delayed degeneration and death of granule cells in the hippocampal dentate gyrus (DG) of the rat brain were studied by means of electron microscopy and a recently elaborated silver method that selectively stains the "dark", collapsed neurons in a Golgi-like manner. At the light microscopic level, the silver technique revealed degenerating granule cells located exclusively in the dentate gyrus; other glucocorticoid receptor-containing regions of the brain were not affected. The silver-stained cell bodies were shrunken, most of the dendrites had a beaded appearance, and the stained axons could be traced along their route to the CA3 pyramidal neurons of the hippocampus. The analysis of 2.5 μm thick Epon-embedded sections stained with toluidine blue revealed hyperchromatic, dark granule neurons and their remains and a heavy glial activity in the vicinity of collapsing neuronal profiles. At the ultrastructural level, early and late stages of neuronal degeneration were observed. The early phase was characterized by markedly increased electron density, a massive shrinkage of the whole somato-dendritic domain, vacuolization of mitochondria, swelling of the nucleolus and condensation of the nuclear chromatin. In the late stage, subcellular organelles were hardly recognizable due to the extremely high electron density and dramatic shrinkage of the cytoplasm. These profiles exhibited disintegration of the cellular organelles and loss of their afferents. Concomitantly, disintegration of granule cell dendrites (clasmatodendrosis) and lifting of "dark" mossy fibers from cell bodies and dendrites of CA3 pyramidal neurons were observed. In the latter cells, this partial denervation caused no apparent signs of ultrastructural alterations. Proliferation of astrocytes and microglial cells was also obvious as they engulfed and eliminated the degenerating neuronal elements. Degenerating neurons frequently occurred adjacent neurons with normal morphology. These morphological features indicate that the delayed degeneration of hippocampal granule cells following adrenalectomy might proceed through a cytoskeletal collapse terminating in cell death.

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