Ultrastructural characterization of the optic pathway in a mouse model of neurofibromatosis-1 optic glioma

K. Y. Kim, W. K. Ju, B. Hegedűs, D. H. Gutmann, M. H. Ellisman

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to investigate the progression of changes in retinal ganglion cells and optic nerve glia in neurofibromatosis-1 (NF1) genetically-engineered mice with optic glioma. Optic glioma tumors were generated in Nf1+/- mice lacking Nf1 expression in GFAP+ cells (astrocytes). Standard immunohistochemistry methods were employed to identify astrocytes (GFAP, S100β), proliferating progenitor cells (sox2, nestin), microglia (Iba1), endothelial cells (CD31) and retinal ganglion cell (RGC) axons (Neurofilament 68k) in Nf1+/-, Nf1GFAPCKO (wild-type mice with Nf1 loss in glial cells), and Nf1+/-GFAPCKO (Nf1+/- mice with Nf1 loss in glial cells) mice. Ultrastructural changes in the optic chiasm and nerve were assessed by electron microscopy (EM). RGC were counted in whole retina preparations using high-resolution, mosaic confocal microscopy following their delineation by retrograde FluoroGold labeling. We found that only Nf1+/-GFAPCKO mice exhibited gross pre-chiasmatic optic nerve and chiasm enlargements containing aggregated GFAP+/nestin+ and S100β+/sox2+ cells (neoplastic glia) as well as increased numbers of blood vessels and microglia. Optic gliomas in Nf1+/-GFAPCKO mice contained axon fiber irregularities and multilamellar bodies of degenerated myelin. EM and EM tomographic analyses showed increased glial disorganization, disoriented axonal projections, profiles of degenerating myelin and structural alterations at nodes of Ranvier. Lastly, we found reduced RGC numbers in Nf1+/-GFAPCKO mice, supporting a model in which the combination of optic nerve Nf1 heterozygosity and glial cell Nf1 loss results in disrupted axonal-glial relationships, subsequently culminating in the degeneration of optic nerve axons and loss of their parent RGC neurons.

Original languageEnglish
Pages (from-to)178-188
Number of pages11
JournalNeuroscience
Volume170
Issue number1
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Optic Nerve Glioma
Neurofibromatosis 1
Neuroglia
Retinal Ganglion Cells
Optic Nerve
Axons
Optic Chiasm
Nestin
Electron Microscopy
Microglia
Myelin Sheath
Astrocytes
Ranvier's Nodes
Intermediate Filaments
Confocal Microscopy
Blood Vessels
Retina
Stem Cells
Endothelial Cells
Cell Count

Keywords

  • Astrocyte
  • Electron microscope tomography
  • Microglial cell
  • Neurofibromatosis-1
  • Optic glioma
  • Retinal ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ultrastructural characterization of the optic pathway in a mouse model of neurofibromatosis-1 optic glioma. / Kim, K. Y.; Ju, W. K.; Hegedűs, B.; Gutmann, D. H.; Ellisman, M. H.

In: Neuroscience, Vol. 170, No. 1, 09.2010, p. 178-188.

Research output: Contribution to journalArticle

Kim, K. Y. ; Ju, W. K. ; Hegedűs, B. ; Gutmann, D. H. ; Ellisman, M. H. / Ultrastructural characterization of the optic pathway in a mouse model of neurofibromatosis-1 optic glioma. In: Neuroscience. 2010 ; Vol. 170, No. 1. pp. 178-188.
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AB - The purpose of this study was to investigate the progression of changes in retinal ganglion cells and optic nerve glia in neurofibromatosis-1 (NF1) genetically-engineered mice with optic glioma. Optic glioma tumors were generated in Nf1+/- mice lacking Nf1 expression in GFAP+ cells (astrocytes). Standard immunohistochemistry methods were employed to identify astrocytes (GFAP, S100β), proliferating progenitor cells (sox2, nestin), microglia (Iba1), endothelial cells (CD31) and retinal ganglion cell (RGC) axons (Neurofilament 68k) in Nf1+/-, Nf1GFAPCKO (wild-type mice with Nf1 loss in glial cells), and Nf1+/-GFAPCKO (Nf1+/- mice with Nf1 loss in glial cells) mice. Ultrastructural changes in the optic chiasm and nerve were assessed by electron microscopy (EM). RGC were counted in whole retina preparations using high-resolution, mosaic confocal microscopy following their delineation by retrograde FluoroGold labeling. We found that only Nf1+/-GFAPCKO mice exhibited gross pre-chiasmatic optic nerve and chiasm enlargements containing aggregated GFAP+/nestin+ and S100β+/sox2+ cells (neoplastic glia) as well as increased numbers of blood vessels and microglia. Optic gliomas in Nf1+/-GFAPCKO mice contained axon fiber irregularities and multilamellar bodies of degenerated myelin. EM and EM tomographic analyses showed increased glial disorganization, disoriented axonal projections, profiles of degenerating myelin and structural alterations at nodes of Ranvier. Lastly, we found reduced RGC numbers in Nf1+/-GFAPCKO mice, supporting a model in which the combination of optic nerve Nf1 heterozygosity and glial cell Nf1 loss results in disrupted axonal-glial relationships, subsequently culminating in the degeneration of optic nerve axons and loss of their parent RGC neurons.

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