Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

Zsolt Liposits, Tamás Görcs, György Sétáló, István Lengvári, Béla Flerkó, Sándor Vigh, Andrew V. Schally

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.

Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalCell And Tissue Research
Volume229
Issue number1
DOIs
Publication statusPublished - Jan 1 1983

Keywords

  • CRF
  • Electron microscopy
  • Median eminence
  • Paraventricular nucleus
  • immunolabelling

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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