Ultrastructural aspects of peptidergic modulation in the peripheral nervous system of Helix pomatia

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The ultrastructural characteristics of peptidergic peripheral contacts in the snail, Helix pomatia, were investigated. With special attention to the innervation of the heart, buccal mass, and salivary gland by Mytilus inhibitory peptide-immunoreactive neurons. Following the application of correlative light- and electron-microscopic pre-embedding immunocytochemistry, the peripheral tissues reveal a rich innervation by Mytilus inhibitory peptide-immunoreactive elements. These neurons establish three types of neuromuscular contacts in the heart and buccal mass: (1) close (16-20 nm) unspecialized membrane contacts; (2) contacts with a relative wide (40-100 nm) intersynaptic cleft; and (3) labeled varicosties located freely in the extracellular space, far (0.5-several μm) from the muscle cells. In the salivary gland, the immunoractive profiles contact both the muscular and glandular elements with close (type 1) and wider (type 2) membrane attachments. The great majority of Mytilus inhibitory peptide-immunoreactive profiles contain an ultrastructurally uniform population of large (120-150 nm) electron dense granules. The ultrastructural features of the innervation by Mytilus inhibitory peptide-immunoreactive elements are compared with those established by immunogold labelled FMRFamide-containing profiles in the heart and salivary gland. These latter display similarities in forming the different kinds of intercellular contacts, and differences in the morphological variability of the content of granules in the immunolabeled profiles. The results suggest diverse, non-synaptic modulatory roles of neuropeptides in the peripheral nervous system of Helix pomatia, including localized membrane effects and neurohormonal-like remote global controls, that may also be of significance in orchestrating the effects of neuropeptides released at the same time on different targets. (C) 2000 Wiley- Liss, Inc.

Original languageEnglish
Pages (from-to)534-546
Number of pages13
JournalMicroscopy Research and Technique
Volume49
Issue number6
DOIs
Publication statusPublished - Jun 15 2000

Fingerprint

peripheral nervous system
Helix pomatia
Mytilus
Peripheral Nervous System
Neurology
salivary glands
helices
Peptides
peptides
innervation
Salivary Glands
Modulation
modulation
Cheek
heart
neuropeptides
profiles
membranes
Neuropeptides
Membranes

Keywords

  • FMRFamide
  • Helix pomatia
  • Immunocytochemistry
  • Mollusc
  • Mytilus inhibitory peptide
  • Periphery
  • Synapses

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

Cite this

Ultrastructural aspects of peptidergic modulation in the peripheral nervous system of Helix pomatia. / Elekes, K.

In: Microscopy Research and Technique, Vol. 49, No. 6, 15.06.2000, p. 534-546.

Research output: Contribution to journalArticle

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