Ultrastructural and immunocytochemical characterization of the rat non-preganglionic Edinger-Westphal nucleus

Diane C W A Van Wijk, Lu Xu, Linda Spiegelberg, Roeland F. Struik, Kari H. Meijer, B. Gaszner, Tamás Kozicz, Eric W. Roubos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The rodent non-preganglionic Edinger-Westphal nucleus (npEW) is involved in the stress adaptation response. Here we describe the ultrastructural organization of this nucleus in the unchallenged rat, using different tissue fixation and embedding methods, and postembedding immunogold labeling. In this way we have (1) identified Ucn1-immunopositive neurons, (2) described the ultrastructure of these neurons with focus on cell organelles involved in secretion (rough endoplasmic reticulum, Golgi apparatus, secretory granules), (3) demonstrated the subcellular coexistence of Ucn1 with cocaine- and amphetamine-related transcript peptide, and (4) classified various morphological types and configurations of synaptic contact present in the npEW and, specifically, on the npEW-Ucn1 neurons. The data obtained provide the morphological basis for future studies on the plastic effects of acute and chronic stressors as well as feeding conditions specifically affecting the secretory activity of npEW-Ucn1 neurons.

Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume164
Issue number1
DOIs
Publication statusPublished - Oct 2009

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neurons
Neurons
rats
Tissue Embedding
Tissue Fixation
amphetamine
cocaine
Rough Endoplasmic Reticulum
secretory granules
rough endoplasmic reticulum
Secretory Vesicles
Golgi Apparatus
Amphetamine
Golgi apparatus
Cocaine
Organelles
Plastics
organelles
Rodentia
ultrastructure

Keywords

  • Cocaine- and amphetamine-regulated transcript peptide
  • Colocalization
  • Postembedding immunogold electron microscopy
  • Stress
  • Synaptology
  • Urocortin 1

ASJC Scopus subject areas

  • Endocrinology

Cite this

Ultrastructural and immunocytochemical characterization of the rat non-preganglionic Edinger-Westphal nucleus. / Van Wijk, Diane C W A; Xu, Lu; Spiegelberg, Linda; Struik, Roeland F.; Meijer, Kari H.; Gaszner, B.; Kozicz, Tamás; Roubos, Eric W.

In: General and Comparative Endocrinology, Vol. 164, No. 1, 10.2009, p. 32-39.

Research output: Contribution to journalArticle

Van Wijk, Diane C W A ; Xu, Lu ; Spiegelberg, Linda ; Struik, Roeland F. ; Meijer, Kari H. ; Gaszner, B. ; Kozicz, Tamás ; Roubos, Eric W. / Ultrastructural and immunocytochemical characterization of the rat non-preganglionic Edinger-Westphal nucleus. In: General and Comparative Endocrinology. 2009 ; Vol. 164, No. 1. pp. 32-39.
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