Ultrastructural and cytochemical study of hepatocytes and lymphocytes during experimental non-A, non-B infections in chimpanzees

Z. Schaff, R. J. Gerety, P. M. Grimley, S. A. Iwarson, D. R. Jackson, E. Tabor

Research output: Contribution to journalArticle

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Abstract

A human agent of non-A, non-B hepatitis (Inoculum I) was transmitted to chimpanzees and alterations in liver and lymphocytes were studied by electron microscopy and by cytochemical techniques during the acute phase of the disease. Three types of cytoplasmic alterations, consisting of a membranous and an amorphous part were observed in the hepatocytes. The density of the amorphous constituent decreased after treatment with pronase, but not after treatment with ribonuclease (RNase) or deoxyribonuclease (DNase). The wall of C-III, but not C-II had fibrils with a periodicity the contrast of which markedly increased after pronase treatment. Cytochemical data suggest that the inclusions (C-I-III) represent a cellular reaction to the infectious agent rather than the virus itself. Intranuclear vermicular inclusions (INI) were observed in hepatocytes and lymphocytes as well, mainly in degenerating cells. Tubuloreticular inclusions (TRS) did not appear in circulating lymphocytes during acute infection; however, they could be induced by human alpha interferon treatment in vitro. Increased numbers of lymphocytes with parallel tubular arrays (PTA) were noted at the peak of serum aminotransferase elevations. The latter two alterations (TRS and PTA) most likely represent immunologic reactions of the host to the infectious agent.

Original languageEnglish
Pages (from-to)25-36
Number of pages12
JournalJournal of Experimental Pathology
Volume2
Issue number1
Publication statusPublished - 1985

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Pan troglodytes
Hepatocytes
Pronase
Lymphocytes
Infection
Intranuclear Inclusion Bodies
Deoxyribonucleases
Lymphocyte Count
Acute Disease
Periodicity
Ribonucleases
Transaminases
Interferon-alpha
Hepatitis
Electron Microscopy
Viruses
Liver
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ultrastructural and cytochemical study of hepatocytes and lymphocytes during experimental non-A, non-B infections in chimpanzees. / Schaff, Z.; Gerety, R. J.; Grimley, P. M.; Iwarson, S. A.; Jackson, D. R.; Tabor, E.

In: Journal of Experimental Pathology, Vol. 2, No. 1, 1985, p. 25-36.

Research output: Contribution to journalArticle

Schaff, Z. ; Gerety, R. J. ; Grimley, P. M. ; Iwarson, S. A. ; Jackson, D. R. ; Tabor, E. / Ultrastructural and cytochemical study of hepatocytes and lymphocytes during experimental non-A, non-B infections in chimpanzees. In: Journal of Experimental Pathology. 1985 ; Vol. 2, No. 1. pp. 25-36.
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