Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses

M. E. Chávez-Delgado, U. Gomez-Pinedo, A. Feria-Velasco, M. Huerta-Viera, S. Castro Castañeda, F. A. López-Dellamary Toral, A. Párducz, S. Luquín-De Anda, J. Mora-Galindo, J. García-Estrada

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Recently, numerous guide chambers for the treatment of injured nerves made up of different biomaterials have been designed, capable of hosting living cells or carrying neurotrophic or neuroactive substances to be directly released to the injured tissue. In this study, chitosan prostheses containing neurosteroids (progesterone and pregnenolone) were used for bridging a 10-mm gap in the rabbit facial nerve. Gas chromatography was used to quantify neurosteroid content in the prostheses prior to and after subcutaneous implantation at different periods of up to 60 days. The regeneration of the nerve fibers were evaluated at 15 and 45 days after axotomy by means of ultrastructural morphometric analysis. Different nerve fibers regenerative patterns were seen depending the groups studied and the analyzed stages. At 15 days after axotomy, the newly regenerating tissue revealed Schwann cells holding nonmyelinated nerve fiber bundles in an incipient and organized regenerative pattern. At 45 days, the regenerating tissue showed myelinated nerve fibers of different sizes, shapes, and myelin sheath thickness. Although the regeneration of the nerve fibers under neurosteroid treatment showed statistically significant differences in comparison with vehicle regenerated tissue, progesterone-loaded chitosan prostheses produced the best guided nerve regeneration response. These findings indicate that chitosan prostheses allowed regeneration of nerve fibers in their lumen, and when containing neurosteroids produced a faster guided nerve regeneration acting as a long-lasting release delivery vehicle.

Original languageEnglish
Pages (from-to)589-600
Number of pages12
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume74
Issue number1
DOIs
Publication statusPublished - Jul 2005

Fingerprint

Pregnenolone
Chitosan
Prosthetics
Progesterone
Neurotransmitter Agents
Fibers
Tissue
Cells
Biocompatible Materials
Biomaterials
Gas chromatography

Keywords

  • Chitosan
  • Drug delivery
  • Facial nerve repairment
  • Guided tissue regeneration
  • Nerve guide
  • Polymers
  • Pregnenolone
  • Progesterone

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Chávez-Delgado, M. E., Gomez-Pinedo, U., Feria-Velasco, A., Huerta-Viera, M., Castro Castañeda, S., López-Dellamary Toral, F. A., ... García-Estrada, J. (2005). Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 74(1), 589-600. https://doi.org/10.1002/jbm.b.30243

Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses. / Chávez-Delgado, M. E.; Gomez-Pinedo, U.; Feria-Velasco, A.; Huerta-Viera, M.; Castro Castañeda, S.; López-Dellamary Toral, F. A.; Párducz, A.; Luquín-De Anda, S.; Mora-Galindo, J.; García-Estrada, J.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 74, No. 1, 07.2005, p. 589-600.

Research output: Contribution to journalArticle

Chávez-Delgado, ME, Gomez-Pinedo, U, Feria-Velasco, A, Huerta-Viera, M, Castro Castañeda, S, López-Dellamary Toral, FA, Párducz, A, Luquín-De Anda, S, Mora-Galindo, J & García-Estrada, J 2005, 'Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses', Journal of Biomedical Materials Research - Part B Applied Biomaterials, vol. 74, no. 1, pp. 589-600. https://doi.org/10.1002/jbm.b.30243
Chávez-Delgado ME, Gomez-Pinedo U, Feria-Velasco A, Huerta-Viera M, Castro Castañeda S, López-Dellamary Toral FA et al. Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses. Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2005 Jul;74(1):589-600. https://doi.org/10.1002/jbm.b.30243
Chávez-Delgado, M. E. ; Gomez-Pinedo, U. ; Feria-Velasco, A. ; Huerta-Viera, M. ; Castro Castañeda, S. ; López-Dellamary Toral, F. A. ; Párducz, A. ; Luquín-De Anda, S. ; Mora-Galindo, J. ; García-Estrada, J. / Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2005 ; Vol. 74, No. 1. pp. 589-600.
@article{1803af3e7d9b480bb9eae76854ce9fad,
title = "Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses",
abstract = "Recently, numerous guide chambers for the treatment of injured nerves made up of different biomaterials have been designed, capable of hosting living cells or carrying neurotrophic or neuroactive substances to be directly released to the injured tissue. In this study, chitosan prostheses containing neurosteroids (progesterone and pregnenolone) were used for bridging a 10-mm gap in the rabbit facial nerve. Gas chromatography was used to quantify neurosteroid content in the prostheses prior to and after subcutaneous implantation at different periods of up to 60 days. The regeneration of the nerve fibers were evaluated at 15 and 45 days after axotomy by means of ultrastructural morphometric analysis. Different nerve fibers regenerative patterns were seen depending the groups studied and the analyzed stages. At 15 days after axotomy, the newly regenerating tissue revealed Schwann cells holding nonmyelinated nerve fiber bundles in an incipient and organized regenerative pattern. At 45 days, the regenerating tissue showed myelinated nerve fibers of different sizes, shapes, and myelin sheath thickness. Although the regeneration of the nerve fibers under neurosteroid treatment showed statistically significant differences in comparison with vehicle regenerated tissue, progesterone-loaded chitosan prostheses produced the best guided nerve regeneration response. These findings indicate that chitosan prostheses allowed regeneration of nerve fibers in their lumen, and when containing neurosteroids produced a faster guided nerve regeneration acting as a long-lasting release delivery vehicle.",
keywords = "Chitosan, Drug delivery, Facial nerve repairment, Guided tissue regeneration, Nerve guide, Polymers, Pregnenolone, Progesterone",
author = "Ch{\'a}vez-Delgado, {M. E.} and U. Gomez-Pinedo and A. Feria-Velasco and M. Huerta-Viera and {Castro Casta{\~n}eda}, S. and {L{\'o}pez-Dellamary Toral}, {F. A.} and A. P{\'a}rducz and {Luqu{\'i}n-De Anda}, S. and J. Mora-Galindo and J. Garc{\'i}a-Estrada",
year = "2005",
month = "7",
doi = "10.1002/jbm.b.30243",
language = "English",
volume = "74",
pages = "589--600",
journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials",
issn = "1552-4973",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Ultrastructural analysis of guided nerve regeneration using progesterone- and pregnenolone-loaded chitosan prostheses

AU - Chávez-Delgado, M. E.

AU - Gomez-Pinedo, U.

AU - Feria-Velasco, A.

AU - Huerta-Viera, M.

AU - Castro Castañeda, S.

AU - López-Dellamary Toral, F. A.

AU - Párducz, A.

AU - Luquín-De Anda, S.

AU - Mora-Galindo, J.

AU - García-Estrada, J.

PY - 2005/7

Y1 - 2005/7

N2 - Recently, numerous guide chambers for the treatment of injured nerves made up of different biomaterials have been designed, capable of hosting living cells or carrying neurotrophic or neuroactive substances to be directly released to the injured tissue. In this study, chitosan prostheses containing neurosteroids (progesterone and pregnenolone) were used for bridging a 10-mm gap in the rabbit facial nerve. Gas chromatography was used to quantify neurosteroid content in the prostheses prior to and after subcutaneous implantation at different periods of up to 60 days. The regeneration of the nerve fibers were evaluated at 15 and 45 days after axotomy by means of ultrastructural morphometric analysis. Different nerve fibers regenerative patterns were seen depending the groups studied and the analyzed stages. At 15 days after axotomy, the newly regenerating tissue revealed Schwann cells holding nonmyelinated nerve fiber bundles in an incipient and organized regenerative pattern. At 45 days, the regenerating tissue showed myelinated nerve fibers of different sizes, shapes, and myelin sheath thickness. Although the regeneration of the nerve fibers under neurosteroid treatment showed statistically significant differences in comparison with vehicle regenerated tissue, progesterone-loaded chitosan prostheses produced the best guided nerve regeneration response. These findings indicate that chitosan prostheses allowed regeneration of nerve fibers in their lumen, and when containing neurosteroids produced a faster guided nerve regeneration acting as a long-lasting release delivery vehicle.

AB - Recently, numerous guide chambers for the treatment of injured nerves made up of different biomaterials have been designed, capable of hosting living cells or carrying neurotrophic or neuroactive substances to be directly released to the injured tissue. In this study, chitosan prostheses containing neurosteroids (progesterone and pregnenolone) were used for bridging a 10-mm gap in the rabbit facial nerve. Gas chromatography was used to quantify neurosteroid content in the prostheses prior to and after subcutaneous implantation at different periods of up to 60 days. The regeneration of the nerve fibers were evaluated at 15 and 45 days after axotomy by means of ultrastructural morphometric analysis. Different nerve fibers regenerative patterns were seen depending the groups studied and the analyzed stages. At 15 days after axotomy, the newly regenerating tissue revealed Schwann cells holding nonmyelinated nerve fiber bundles in an incipient and organized regenerative pattern. At 45 days, the regenerating tissue showed myelinated nerve fibers of different sizes, shapes, and myelin sheath thickness. Although the regeneration of the nerve fibers under neurosteroid treatment showed statistically significant differences in comparison with vehicle regenerated tissue, progesterone-loaded chitosan prostheses produced the best guided nerve regeneration response. These findings indicate that chitosan prostheses allowed regeneration of nerve fibers in their lumen, and when containing neurosteroids produced a faster guided nerve regeneration acting as a long-lasting release delivery vehicle.

KW - Chitosan

KW - Drug delivery

KW - Facial nerve repairment

KW - Guided tissue regeneration

KW - Nerve guide

KW - Polymers

KW - Pregnenolone

KW - Progesterone

UR - http://www.scopus.com/inward/record.url?scp=21444441396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21444441396&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.30243

DO - 10.1002/jbm.b.30243

M3 - Article

VL - 74

SP - 589

EP - 600

JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials

SN - 1552-4973

IS - 1

ER -