Ultrasonographic appearance of the conceptus, fetal heart rate and profiles of pregnancy-associated glycoproteins (PAG) and prostaglandin F -metabolite (PGF-metabolite) after induction of fetal death with aglepristone during early gestation in cattle

Simone P. Breukelman, O. Szenci, Jean Francois Beckers, Hans Kindahl, Edu J H Mulder, F. Herman Jonker, Bert Van Der Weijden, Dénes Revy, Károly Pogany, José Sulon, István Némedi, Marcel A M Taverne

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A higher incidence of fetal losses, especially after the use of artificial reproduction techniques, asks for more intensive monitoring of bovine pregnancies. In this study, a model for fetal death (FD) was created by administering the antiprogesterone aglepristone twice, at Day 47 and 48 of gestation (n = 5). Control heifers received the solvent (n = 5). The temporal relationships between changes in ultrasonographic appearance of fetal fluids and membranes, fetal heart rate (FHR) and peripheral plasma levels of pregnancy-associated glycoprotein (PAG) and PGF2α-metabolite as determined by radioimmunoassay associated with FD were monitored at eight hour intervals around treatment. For the analysis of plasma levels the period under study was divided into five epochs (T1: before injection of aglepristone/solvent; T2: from first to second injection; T3: from second injection to FD; T4: from diagnosis of FD to 56 h later; T5: from 56 h to 104 h after diagnosis of FD). Control heifers produced healthy calves at term, but in treated heifers, FD occurred on average at 58 (range 48-80) h after first injection of aglepristone. Fetal death was always preceded by a visible reduction of the amount of allantoic fluid and by segregation of the allantochorionic membrane from the endometrium. FHR remained rather constant in both groups, but a (non-significant) drop in FHR around 8 h before FD was diagnosed in four of five treated animals. All fetuses were expulsed after FD. Levels of PAG remained constant or even slightly increased in controls, but decreased in treated animals from T2 onward: levels during T4 and T5 significantly differed from those during T1 and from values in controls during T4 and T5 (P <0.01). PGF2α-metabolite levels did not change in the controls, but in the treated group they were significantly higher during T3 when compared to T1 (P <0.05). After this increase, a sharp decrease in PGF2α-metabolite level occurred, reaching a significantly lower level at T5 when compared to control animals (P = 0.01). It is concluded, that FD induced by aglepristone is preceded by ultrasonographic visible changes in fetal membranes and fluids and a rise in PGF2α-metabolite and is followed by a drop in PAG and PGF2α-metabolite.

Original languageEnglish
Pages (from-to)917-933
Number of pages17
JournalTheriogenology
Volume64
Issue number4
DOIs
Publication statusPublished - Sep 1 2005

Fingerprint

Fetal Heart Rate
conceptus
Fetal Death
fetal death
Prostaglandins F
prostaglandins
heart rate
glycoproteins
Glycoproteins
pregnancy
metabolites
Pregnancy
cattle
injection
Extraembryonic Membranes
extraembryonic membranes
heifers
Injections
aglepristone
allantoic fluid

Keywords

  • Aglepristone
  • Fetal death
  • Fetal heart rate
  • PGF- metabolite
  • Pregnancy-associated glycoproteins

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Ultrasonographic appearance of the conceptus, fetal heart rate and profiles of pregnancy-associated glycoproteins (PAG) and prostaglandin F -metabolite (PGF-metabolite) after induction of fetal death with aglepristone during early gestation in cattle. / Breukelman, Simone P.; Szenci, O.; Beckers, Jean Francois; Kindahl, Hans; Mulder, Edu J H; Jonker, F. Herman; Van Der Weijden, Bert; Revy, Dénes; Pogany, Károly; Sulon, José; Némedi, István; Taverne, Marcel A M.

In: Theriogenology, Vol. 64, No. 4, 01.09.2005, p. 917-933.

Research output: Contribution to journalArticle

Breukelman, Simone P. ; Szenci, O. ; Beckers, Jean Francois ; Kindahl, Hans ; Mulder, Edu J H ; Jonker, F. Herman ; Van Der Weijden, Bert ; Revy, Dénes ; Pogany, Károly ; Sulon, José ; Némedi, István ; Taverne, Marcel A M. / Ultrasonographic appearance of the conceptus, fetal heart rate and profiles of pregnancy-associated glycoproteins (PAG) and prostaglandin F -metabolite (PGF-metabolite) after induction of fetal death with aglepristone during early gestation in cattle. In: Theriogenology. 2005 ; Vol. 64, No. 4. pp. 917-933.
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AU - Breukelman, Simone P.

AU - Szenci, O.

AU - Beckers, Jean Francois

AU - Kindahl, Hans

AU - Mulder, Edu J H

AU - Jonker, F. Herman

AU - Van Der Weijden, Bert

AU - Revy, Dénes

AU - Pogany, Károly

AU - Sulon, José

AU - Némedi, István

AU - Taverne, Marcel A M

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N2 - A higher incidence of fetal losses, especially after the use of artificial reproduction techniques, asks for more intensive monitoring of bovine pregnancies. In this study, a model for fetal death (FD) was created by administering the antiprogesterone aglepristone twice, at Day 47 and 48 of gestation (n = 5). Control heifers received the solvent (n = 5). The temporal relationships between changes in ultrasonographic appearance of fetal fluids and membranes, fetal heart rate (FHR) and peripheral plasma levels of pregnancy-associated glycoprotein (PAG) and PGF2α-metabolite as determined by radioimmunoassay associated with FD were monitored at eight hour intervals around treatment. For the analysis of plasma levels the period under study was divided into five epochs (T1: before injection of aglepristone/solvent; T2: from first to second injection; T3: from second injection to FD; T4: from diagnosis of FD to 56 h later; T5: from 56 h to 104 h after diagnosis of FD). Control heifers produced healthy calves at term, but in treated heifers, FD occurred on average at 58 (range 48-80) h after first injection of aglepristone. Fetal death was always preceded by a visible reduction of the amount of allantoic fluid and by segregation of the allantochorionic membrane from the endometrium. FHR remained rather constant in both groups, but a (non-significant) drop in FHR around 8 h before FD was diagnosed in four of five treated animals. All fetuses were expulsed after FD. Levels of PAG remained constant or even slightly increased in controls, but decreased in treated animals from T2 onward: levels during T4 and T5 significantly differed from those during T1 and from values in controls during T4 and T5 (P <0.01). PGF2α-metabolite levels did not change in the controls, but in the treated group they were significantly higher during T3 when compared to T1 (P <0.05). After this increase, a sharp decrease in PGF2α-metabolite level occurred, reaching a significantly lower level at T5 when compared to control animals (P = 0.01). It is concluded, that FD induced by aglepristone is preceded by ultrasonographic visible changes in fetal membranes and fluids and a rise in PGF2α-metabolite and is followed by a drop in PAG and PGF2α-metabolite.

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