Ubiquiter circovirus sequences raise challenges in laboratory diagnosis: The case of honey bee and bee mite, reptiles, and free living amoebae

Szilvia Marton, Katalin Ihász, György Lengyel, Szilvia L. Farkas, A. Dán, Petra Paulus, K. Bányai, Eniko Fehér

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Circoviruses of pigs and birds are established pathogens, however, the exact role of other, recently described circoviruses and circovirus-like viruses remains to be elucidated. The aim of this study was the detection of circoviruses in neglected host species, including honey bees, exotic reptiles and free-living amoebae by widely used broad-spectrum polymerase chain reaction (PCR) assays specific for the replication initiation protein coding gene of these viruses. The majority of sequences obtained from honey bees were highly similar to canine and porcine circoviruses, or, were distantly related to dragonfly cycloviruses. Other rep sequences detected in some honey bees, reptiles and amoebae showed similarities to various rep sequences deposited in the GenBank. Back-to-back PCR primers designed for the amplification of whole viral genomes failed to work that suggested the existence of integrated rep-like elements in many samples. Rolling circle amplification and exonuclease treatment confirmed the absence of small circular DNA genomes in the specimens analysed. In case of honey bees Varroa mite DNA contamination might be a source of the identified endogenous rep-like elements. The reptile and amoebae rep-like sequences were nearly identical with each other and with sequences detected in chimpanzee feces raising the possibility that detection of novel or unusual rep-like elements in some host species might originate from the microbial community of the host. Our results indicate that attention is needed when broad-spectrum rep gene specific polymerase chain reaction is chosen for laboratory diagnosis of circovirus infections.

Original languageEnglish
Pages (from-to)57-73
Number of pages17
JournalActa Microbiologica et Immunologica Hungarica
Volume62
Issue number1
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Circovirus
Amoeba
Reptiles
Honey
Clinical Laboratory Techniques
Mites
Bees
Polymerase Chain Reaction
Circoviridae Infections
Varroidae
DNA Contamination
Odonata
Viruses
Circular DNA
Exonucleases
Pan troglodytes
Viral Genome
Nucleic Acid Databases
Feces
Birds

Keywords

  • Circovirus
  • Integrated
  • Rep
  • Sequencing

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Ubiquiter circovirus sequences raise challenges in laboratory diagnosis : The case of honey bee and bee mite, reptiles, and free living amoebae. / Marton, Szilvia; Ihász, Katalin; Lengyel, György; Farkas, Szilvia L.; Dán, A.; Paulus, Petra; Bányai, K.; Fehér, Eniko.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 62, No. 1, 01.03.2015, p. 57-73.

Research output: Contribution to journalArticle

Marton, Szilvia ; Ihász, Katalin ; Lengyel, György ; Farkas, Szilvia L. ; Dán, A. ; Paulus, Petra ; Bányai, K. ; Fehér, Eniko. / Ubiquiter circovirus sequences raise challenges in laboratory diagnosis : The case of honey bee and bee mite, reptiles, and free living amoebae. In: Acta Microbiologica et Immunologica Hungarica. 2015 ; Vol. 62, No. 1. pp. 57-73.
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