Tyrosine hydroxylase-negative, dopaminergic neurons are targets for transmitter-depleting action of haloperidol in the snail brain

Dmitri A. Sakharov, Elena E. Voronezhskaya, Leonid Nezlin, Michael W. Baker, K. Elekes, Roger P. Croll

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

1. The effects of long term administration of micromolar concentrations of the D2 antagonist haloperidol upon monoaminergic neurons in the snail Lymnaea stagnalis was investigated. 2. Treatment by bath application with 0.5-2.0 micromolar haloperidol, caused a significant, continuous depletion of dopamine levels in the nervous system as revealed by high performance liquid chromatography. 3. A transient depletion of serotonin was also observed, but DOPA and norepinephrine levels were unaffected. Similar depletion of dopamine was observed after the land snail, Achatina fulica, was injected with haloperidol on each of 4 consecutive days. 4. The depletion of dopamine as revealed with glyoxylate-induced fluorescence in Lymnaea appears to be restricted to a subpopulation of catecholaminergic neurons which are immuno- negative for tyrosine hydroxylase, the synthetic enzyme responsible for the conversion of tyrosine to DOPA. 5. The results thus demonstrate a depleting action of low micromolar doses of chronic haloperidol on specific subsets of dopaminergic neurons and a novel preparation for studying catecholaminergic mechanisms operating across the animal kingdom.

Original languageEnglish
Pages (from-to)451-461
Number of pages11
JournalCellular and Molecular Neurobiology
Volume16
Issue number4
Publication statusPublished - 1996

Fingerprint

Dopaminergic Neurons
Snails
Tyrosine 3-Monooxygenase
Haloperidol
Neurons
Transmitters
Brain
Lymnaea
Dopamine
High performance liquid chromatography
Neurology
Baths
Nervous System
Tyrosine
Serotonin
Norepinephrine
Animals
Fluorescence
High Pressure Liquid Chromatography
Enzymes

Keywords

  • antipsychotic
  • catecholamine
  • gastropod
  • monoamine
  • neuroleptic
  • serotonin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Tyrosine hydroxylase-negative, dopaminergic neurons are targets for transmitter-depleting action of haloperidol in the snail brain. / Sakharov, Dmitri A.; Voronezhskaya, Elena E.; Nezlin, Leonid; Baker, Michael W.; Elekes, K.; Croll, Roger P.

In: Cellular and Molecular Neurobiology, Vol. 16, No. 4, 1996, p. 451-461.

Research output: Contribution to journalArticle

Sakharov, Dmitri A. ; Voronezhskaya, Elena E. ; Nezlin, Leonid ; Baker, Michael W. ; Elekes, K. ; Croll, Roger P. / Tyrosine hydroxylase-negative, dopaminergic neurons are targets for transmitter-depleting action of haloperidol in the snail brain. In: Cellular and Molecular Neurobiology. 1996 ; Vol. 16, No. 4. pp. 451-461.
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