Tyrosine hydroxylase in magnocellular neurosecretory neurons: Response to physiological manipulations

J. Kiss, Éva Mezey

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Tyrosine hydroxylase (TH) immunoreactivity is present in a few magnocellular neurons in the supraoptic and paraventricular nuclei of normal Sprague-Dawley and heterozygous Brattleboro rats. Manipulations commonly used to enhance immunostaining of cell bodies, such as colchicine treatment or lesioning of efferent fibers, induced only a slight increase in TH of magnocellular neurons in Sprague-Dawley rats. Seven days after bilateral midbrain transections, which destroyed ascending afferents to paraventricular and supraoptic nuclei from the lower brainstem, there was a substantial increase in TH immunoreactivity in magnocellular neurons. The lesions resulted in a marked increase in the intensity of the staining and in the number of stained cells. Similar increases were induced by giving animals 2% NaCl to drink for 5 days. Neurons in the supraoptic and paraventricular nuclei of homozygous Brattleboro rats show markedly more TH staining than those of normal rats. Vasopressin treatment lowered TH immunoreactivity in magnocellular neurons of Brattleboro rats to normal levels. These observations suggest that extracellular factors, probably afferent inputs, regulate the level of TH immunoreactivity in magnocellular neurons.

Original languageEnglish
Pages (from-to)519-525
Number of pages7
JournalNeuroendocrinology
Volume43
Issue number4
DOIs
Publication statusPublished - 1986

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Tyrosine 3-Monooxygenase
Brattleboro Rats
Neurons
Supraoptic Nucleus
Paraventricular Hypothalamic Nucleus
Staining and Labeling
Colchicine
Mesencephalon
Vasopressins
Brain Stem
Sprague Dawley Rats
Cell Count

Keywords

  • Brattleboro rat
  • Paraventricular nucleus
  • Supraoptic nucleus

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Tyrosine hydroxylase in magnocellular neurosecretory neurons : Response to physiological manipulations. / Kiss, J.; Mezey, Éva.

In: Neuroendocrinology, Vol. 43, No. 4, 1986, p. 519-525.

Research output: Contribution to journalArticle

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