Type X collagen in human enamel development: A possible role in mineralization

Szabolcs Felszeghy, Krisztina Holló, László Módis, Mikko J. Lammi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Although type X collagen is one of the key molecules in endochondral ossification, no data are available on whether it is present in dental structures when mineralization is proceeding. We therefore monitored the appearance of type X collagen in tooth germs of human samples ranging in gestational age from 17-week-old fetuses to 9-week-old newborn. Using immunohistochemistry, ELISA techniques, and Western blotting, we show that type X collagen is present in human tooth germ during enamel maturation. Intense immunohistochemical staining for collagen type X was observed in the enamel and in the apical parts of secretory ameloblast at the bell stage when the dentine and enamel matrix were already under formation. The odontoblasts, the dentine, and the pulp were not stained. In the early (9-week) postnatal stage, the staining for collagen type X in the enamel matrix was diminished, and only a very weak signal could be detected in the secretory ameloblasts. A positive reaction for collagen type X was also observed in ELISA assay of extracts obtained from human embryonic enamel and hypertrophic cartilage samples. The Western blot analysis of the enamel demonstrated that size of the molecule detected by MoAb X53 is characteristic of the type X collagen. This correlates well with our immunohistochemical findings. Based on these data, we propose that type X collagen is one of the candidate molecules present in the enamel matrix that might be involved in mineralization of the enamel.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalActa Odontologica Scandinavica
Volume58
Issue number4
DOIs
Publication statusPublished - Aug 2000

Keywords

  • Collagen type X
  • Enamel mineralization
  • Human tooth development

ASJC Scopus subject areas

  • Dentistry(all)

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