Type X collagen in human enamel development

A possible role in mineralization

S. Felszeghy, Krisztina Holló, László Módis, Mikko J. Lammi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Although type X collagen is one of the key molecules in endochondral ossification, no data are available on whether it is present in dental structures when mineralization is proceeding. We therefore monitored the appearance of type X collagen in tooth germs of human samples ranging in gestational age from 17-week-old fetuses to 9-week-old newborn. Using immunohistochemistry, ELISA techniques, and Western blotting, we show that type X collagen is present in human tooth germ during enamel maturation. Intense immunohistochemical staining for collagen type X was observed in the enamel and in the apical parts of secretory ameloblast at the bell stage when the dentine and enamel matrix were already under formation. The odontoblasts, the dentine, and the pulp were not stained. In the early (9-week) postnatal stage, the staining for collagen type X in the enamel matrix was diminished, and only a very weak signal could be detected in the secretory ameloblasts. A positive reaction for collagen type X was also observed in ELISA assay of extracts obtained from human embryonic enamel and hypertrophic cartilage samples. The Western blot analysis of the enamel demonstrated that size of the molecule detected by MoAb X53 is characteristic of the type X collagen. This correlates well with our immunohistochemical findings. Based on these data, we propose that type X collagen is one of the candidate molecules present in the enamel matrix that might be involved in mineralization of the enamel.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalActa Odontologica Scandinavica
Volume58
Issue number4
Publication statusPublished - Aug 2000

Fingerprint

Collagen Type X
Human Development
Dental Enamel
Tooth Germ
Ameloblasts
Dentin
Western Blotting
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Odontoblasts
Osteogenesis
Gestational Age
Cartilage
Tooth
Fetus
Immunohistochemistry

Keywords

  • Collagen type X
  • Enamel mineralization
  • Human tooth development

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Type X collagen in human enamel development : A possible role in mineralization. / Felszeghy, S.; Holló, Krisztina; Módis, László; Lammi, Mikko J.

In: Acta Odontologica Scandinavica, Vol. 58, No. 4, 08.2000, p. 171-176.

Research output: Contribution to journalArticle

Felszeghy, S. ; Holló, Krisztina ; Módis, László ; Lammi, Mikko J. / Type X collagen in human enamel development : A possible role in mineralization. In: Acta Odontologica Scandinavica. 2000 ; Vol. 58, No. 4. pp. 171-176.
@article{475694c37bd54b83a3488c0c1f9a48cc,
title = "Type X collagen in human enamel development: A possible role in mineralization",
abstract = "Although type X collagen is one of the key molecules in endochondral ossification, no data are available on whether it is present in dental structures when mineralization is proceeding. We therefore monitored the appearance of type X collagen in tooth germs of human samples ranging in gestational age from 17-week-old fetuses to 9-week-old newborn. Using immunohistochemistry, ELISA techniques, and Western blotting, we show that type X collagen is present in human tooth germ during enamel maturation. Intense immunohistochemical staining for collagen type X was observed in the enamel and in the apical parts of secretory ameloblast at the bell stage when the dentine and enamel matrix were already under formation. The odontoblasts, the dentine, and the pulp were not stained. In the early (9-week) postnatal stage, the staining for collagen type X in the enamel matrix was diminished, and only a very weak signal could be detected in the secretory ameloblasts. A positive reaction for collagen type X was also observed in ELISA assay of extracts obtained from human embryonic enamel and hypertrophic cartilage samples. The Western blot analysis of the enamel demonstrated that size of the molecule detected by MoAb X53 is characteristic of the type X collagen. This correlates well with our immunohistochemical findings. Based on these data, we propose that type X collagen is one of the candidate molecules present in the enamel matrix that might be involved in mineralization of the enamel.",
keywords = "Collagen type X, Enamel mineralization, Human tooth development",
author = "S. Felszeghy and Krisztina Holl{\'o} and L{\'a}szl{\'o} M{\'o}dis and Lammi, {Mikko J.}",
year = "2000",
month = "8",
language = "English",
volume = "58",
pages = "171--176",
journal = "Acta Odontologica Scandinavica",
issn = "0001-6357",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Type X collagen in human enamel development

T2 - A possible role in mineralization

AU - Felszeghy, S.

AU - Holló, Krisztina

AU - Módis, László

AU - Lammi, Mikko J.

PY - 2000/8

Y1 - 2000/8

N2 - Although type X collagen is one of the key molecules in endochondral ossification, no data are available on whether it is present in dental structures when mineralization is proceeding. We therefore monitored the appearance of type X collagen in tooth germs of human samples ranging in gestational age from 17-week-old fetuses to 9-week-old newborn. Using immunohistochemistry, ELISA techniques, and Western blotting, we show that type X collagen is present in human tooth germ during enamel maturation. Intense immunohistochemical staining for collagen type X was observed in the enamel and in the apical parts of secretory ameloblast at the bell stage when the dentine and enamel matrix were already under formation. The odontoblasts, the dentine, and the pulp were not stained. In the early (9-week) postnatal stage, the staining for collagen type X in the enamel matrix was diminished, and only a very weak signal could be detected in the secretory ameloblasts. A positive reaction for collagen type X was also observed in ELISA assay of extracts obtained from human embryonic enamel and hypertrophic cartilage samples. The Western blot analysis of the enamel demonstrated that size of the molecule detected by MoAb X53 is characteristic of the type X collagen. This correlates well with our immunohistochemical findings. Based on these data, we propose that type X collagen is one of the candidate molecules present in the enamel matrix that might be involved in mineralization of the enamel.

AB - Although type X collagen is one of the key molecules in endochondral ossification, no data are available on whether it is present in dental structures when mineralization is proceeding. We therefore monitored the appearance of type X collagen in tooth germs of human samples ranging in gestational age from 17-week-old fetuses to 9-week-old newborn. Using immunohistochemistry, ELISA techniques, and Western blotting, we show that type X collagen is present in human tooth germ during enamel maturation. Intense immunohistochemical staining for collagen type X was observed in the enamel and in the apical parts of secretory ameloblast at the bell stage when the dentine and enamel matrix were already under formation. The odontoblasts, the dentine, and the pulp were not stained. In the early (9-week) postnatal stage, the staining for collagen type X in the enamel matrix was diminished, and only a very weak signal could be detected in the secretory ameloblasts. A positive reaction for collagen type X was also observed in ELISA assay of extracts obtained from human embryonic enamel and hypertrophic cartilage samples. The Western blot analysis of the enamel demonstrated that size of the molecule detected by MoAb X53 is characteristic of the type X collagen. This correlates well with our immunohistochemical findings. Based on these data, we propose that type X collagen is one of the candidate molecules present in the enamel matrix that might be involved in mineralization of the enamel.

KW - Collagen type X

KW - Enamel mineralization

KW - Human tooth development

UR - http://www.scopus.com/inward/record.url?scp=0034252447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034252447&partnerID=8YFLogxK

M3 - Article

VL - 58

SP - 171

EP - 176

JO - Acta Odontologica Scandinavica

JF - Acta Odontologica Scandinavica

SN - 0001-6357

IS - 4

ER -