Type A and B gaba receptors mediate inhibition of acetylcholine release from cholinergic nerve terminals in the superior cervical ganglion of rat

Z. Farkas, P. Kása, V. J. Balcar, F. Joó, J. R. Wolff

Research output: Contribution to journalArticle

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Abstract

The effects of γ-amino-n-butyric acid (GABA), (+)bicuculline, isoguvacine and 3-(4-chlorophenyl)-4-aminobutyrate [(±)baclofen] on the K-induced release of [3H]acetylcholine (ACh) were studied in the superior cervical ganglia of the rat in vitro. GABA and isoguvacine inhibited [3H]ACh release and these inhibitions were reversible by (+)bicuculline. Furthermore, the release of [3H]ACh was also inhibited by (±)baclofen. In receptor-binding studies, binding of [3H]GABA to membrane preparations from the superior cervical ganglia was inhibited by both (±)baclofen and (+)bicuculline. It is concluded that the inhibitory effect of GABA on the release of ACh can be mediated by GABAA(bicuculline-sensitive) and by GABAB (baclofen-activated) receptors. Our findings are compatible with the existence of a non-synaptic GABAergic inhibitory system involving GABAA and GABAB receptors on cholinergic nerve terminals in the superior cervical ganglion of rat.

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalNeurochemistry International
Volume8
Issue number4
DOIs
Publication statusPublished - 1986

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Superior Cervical Ganglion
Bicuculline
gamma-Aminobutyric Acid
Cholinergic Agents
Baclofen
Acetylcholine
GABA-B Receptors
Aminobutyrates
Butyric Acid
GABA-A Receptors
Membranes
isoguvacine

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Type A and B gaba receptors mediate inhibition of acetylcholine release from cholinergic nerve terminals in the superior cervical ganglion of rat. / Farkas, Z.; Kása, P.; Balcar, V. J.; Joó, F.; Wolff, J. R.

In: Neurochemistry International, Vol. 8, No. 4, 1986, p. 565-572.

Research output: Contribution to journalArticle

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AU - Wolff, J. R.

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