Two successful pregnancies obtained following oocyte vitrification and embryo re-vitrification

Ching Chien Chang, Daniel B. Shapiro, Diana Patricia Bernal, Graham Wright, Hilton I. Kort, P. Nagy

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Recent clinical reports not only show that cryopreserved embryos can be successfully used for human fertility treatment, but also that cryopreserved oocytes may be used successfully as an adjunct to human assisted reproductive technologies. Vitrification is known to establish a glass-like solid state during the cooling process. The high concentration of cryoprotectants and an extremely rapid rate of cooling are responsible for the formation of the solid state, and also prevent formation of intracellular ice crystals. Hence, in theory, vitrification should minimize cryo-injuries, and therefore has great promise for oocyte and embryo cryopreservation. This article describes two pregnancies from vitrified - warmed blastocysts obtained after intracytoplasmic sperm injection fertilization of vitrified - warmed oocytes. Vitrification was employed to cryopreserve the oocytes and the subsequent blastocysts. The results present the intriguing implication that vitrification may serve as an efficient method for clinical oocyte cryopreservation and embryo re-cryopreservation.

Original languageEnglish
Pages (from-to)346-349
Number of pages4
JournalReproductive BioMedicine Online
Volume16
Issue number3
Publication statusPublished - Mar 2008

Fingerprint

Vitrification
Oocytes
Embryonic Structures
Cryopreservation
Pregnancy
Blastocyst
Assisted Reproductive Techniques
Intracytoplasmic Sperm Injections
Ice
Fertilization
Glass
Fertility
Wounds and Injuries

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Chang, C. C., Shapiro, D. B., Bernal, D. P., Wright, G., Kort, H. I., & Nagy, P. (2008). Two successful pregnancies obtained following oocyte vitrification and embryo re-vitrification. Reproductive BioMedicine Online, 16(3), 346-349.

Two successful pregnancies obtained following oocyte vitrification and embryo re-vitrification. / Chang, Ching Chien; Shapiro, Daniel B.; Bernal, Diana Patricia; Wright, Graham; Kort, Hilton I.; Nagy, P.

In: Reproductive BioMedicine Online, Vol. 16, No. 3, 03.2008, p. 346-349.

Research output: Contribution to journalArticle

Chang, CC, Shapiro, DB, Bernal, DP, Wright, G, Kort, HI & Nagy, P 2008, 'Two successful pregnancies obtained following oocyte vitrification and embryo re-vitrification', Reproductive BioMedicine Online, vol. 16, no. 3, pp. 346-349.
Chang, Ching Chien ; Shapiro, Daniel B. ; Bernal, Diana Patricia ; Wright, Graham ; Kort, Hilton I. ; Nagy, P. / Two successful pregnancies obtained following oocyte vitrification and embryo re-vitrification. In: Reproductive BioMedicine Online. 2008 ; Vol. 16, No. 3. pp. 346-349.
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