Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy

Keiichi Inoue, N. Bokor, Satoshi Kogure, Masaaki Fujii, Makoto Sakai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

IR absorption of chemical species in microscopic objects such as biological cells cannot be measured by conventional IR microscopes, because of their low resolution. To overcome this problem, we developed a novel far-field IR super-resolution microscope employing transient fluorescence detected IR spectroscopy. The resolution of this microscope was shown to be 880 nm by measuring the image of 1 μm fluorescent beads. Furthermore, it succeeded in resolving beads located 1.4 μm apart from each other. This is considerably smaller than the diffraction limit of the applied IR light (3.4 μm). These results suggest the capability of our microscope to study sub-micron targets such as sub-cellular structures of biological cells.

Original languageEnglish
Pages (from-to)12013-12018
Number of pages6
JournalOptics Express
Volume17
Issue number14
DOIs
Publication statusPublished - Jul 6 2009

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microscopes
fluorescence
beads
spectroscopy
far fields
diffraction

Keywords

  • (110.3080) Infrared imaging
  • (130.3060) Infrared
  • (170.0110) Imaging system
  • (180.0180) Microscopy
  • (180.2520) Fluorescence microscopy
  • (320.5390) Picosecond phenomena

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy. / Inoue, Keiichi; Bokor, N.; Kogure, Satoshi; Fujii, Masaaki; Sakai, Makoto.

In: Optics Express, Vol. 17, No. 14, 06.07.2009, p. 12013-12018.

Research output: Contribution to journalArticle

Inoue, Keiichi ; Bokor, N. ; Kogure, Satoshi ; Fujii, Masaaki ; Sakai, Makoto. / Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy. In: Optics Express. 2009 ; Vol. 17, No. 14. pp. 12013-12018.
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