Two-dimensional thin-layer chromatography of plant ecdysteroids

M. Báthori, G. Blunden, H. Kalász

Research output: Contribution to journalArticle

19 Citations (Scopus)


Two-dimensional thin-layer chromatography has been used to screen plant samples for ecdysteroids. Satisfactory separations were obtained even in the presence of substantial impurities and for mixtures containing many ecdysteroids. Normal-phase silica and octadecyl silica plates were used to differentiate apolar and polar ecdysteroids, respectively. The major advantages of thin-layer chromatography are multiple detection, which enables specific detection of the ecdysteroids. Use of a small amount of water in the mobile phase in the first dimension, then a water-free mobile phase in the second dimension, can afford favorable separations.

Original languageEnglish
Pages (from-to)815-817
Number of pages3
Issue number11-12
Publication statusPublished - Jan 1 2000


  • Ecdysteroids
  • Thin-layer chromatography
  • Two-dimensional chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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