Two-dimensional receptor patterns in the plasma membrane of cells. A critical evaluation of their identification, origin and information content

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Abstract

A concise review is presented on the nature, possible origin and functional significance of cell surface receptor patterns in the plasma membrane of lymphoid cells. A special emphasize has been laid on the available methodological approaches, their individual virtues and sources of errors. Fluorescence energy transfer is one of the oldest available means for studying non-randomized co-distribution patterns of cell surface receptors. A detailed and critical description is given on the generation of two-dimensional cell surface receptor patterns based on pair-wise energy transfer measurements. A second hierarchical-level of receptor clusters have been described by electron and scanning force microscopies after immuno-gold-labeling of distinct receptor kinds. The origin of these receptor islands at a nanometer scale and island groups at a higher hierarchical (μm) level, has been explained mostly by detergent insoluble glycolipid-enriched complexes known as rafts, or detergent insoluble glycolipids (DIGs). These rafts are the most-likely organizational forces behind at least some kind of receptor clustering [K. Simons et al., Nature 387 (1997) 569]. These models, which have great significance in trans-membrane signaling and intra-membrane and intracellular trafficking, are accentuating the necessity to revisit the Singer-Nicolson fluid mosaic membrane model and substitute the free protein diffusion with a restricted diffusion concept [S.J. Singer et al., Science 175 (1972) 720]. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalBiophysical Chemistry
Volume82
Issue number2-3
DOIs
Publication statusPublished - Dec 13 1999

Fingerprint

Cell Surface Receptors
Cell membranes
Plasma Cells
Glycolipids
Energy Transfer
Cell Membrane
membranes
Membranes
Islands
Detergents
Energy transfer
evaluation
cells
Singing
Intracellular Membranes
Atomic Force Microscopy
rafts
detergents
Gold
Electron Scanning Microscopy

Keywords

  • Cell-surface organization
  • Electron microscopy
  • Fluorescence energy transfer
  • Receptor patterns
  • Scanning force microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Physical and Theoretical Chemistry

Cite this

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title = "Two-dimensional receptor patterns in the plasma membrane of cells. A critical evaluation of their identification, origin and information content",
abstract = "A concise review is presented on the nature, possible origin and functional significance of cell surface receptor patterns in the plasma membrane of lymphoid cells. A special emphasize has been laid on the available methodological approaches, their individual virtues and sources of errors. Fluorescence energy transfer is one of the oldest available means for studying non-randomized co-distribution patterns of cell surface receptors. A detailed and critical description is given on the generation of two-dimensional cell surface receptor patterns based on pair-wise energy transfer measurements. A second hierarchical-level of receptor clusters have been described by electron and scanning force microscopies after immuno-gold-labeling of distinct receptor kinds. The origin of these receptor islands at a nanometer scale and island groups at a higher hierarchical (μm) level, has been explained mostly by detergent insoluble glycolipid-enriched complexes known as rafts, or detergent insoluble glycolipids (DIGs). These rafts are the most-likely organizational forces behind at least some kind of receptor clustering [K. Simons et al., Nature 387 (1997) 569]. These models, which have great significance in trans-membrane signaling and intra-membrane and intracellular trafficking, are accentuating the necessity to revisit the Singer-Nicolson fluid mosaic membrane model and substitute the free protein diffusion with a restricted diffusion concept [S.J. Singer et al., Science 175 (1972) 720]. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "Cell-surface organization, Electron microscopy, Fluorescence energy transfer, Receptor patterns, Scanning force microscopy",
author = "S. Damjanovich and L. Bene and J. Matk{\'o} and L. M{\'a}tyus and Z. Krasznai and G{\'a}bor Szab{\'o} and Carlo Pieri and R. G{\'a}sp{\'a}r and J. Sz{\"o}llősi",
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AU - Damjanovich, S.

AU - Bene, L.

AU - Matkó, J.

AU - Mátyus, L.

AU - Krasznai, Z.

AU - Szabó, Gábor

AU - Pieri, Carlo

AU - Gáspár, R.

AU - Szöllősi, J.

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AB - A concise review is presented on the nature, possible origin and functional significance of cell surface receptor patterns in the plasma membrane of lymphoid cells. A special emphasize has been laid on the available methodological approaches, their individual virtues and sources of errors. Fluorescence energy transfer is one of the oldest available means for studying non-randomized co-distribution patterns of cell surface receptors. A detailed and critical description is given on the generation of two-dimensional cell surface receptor patterns based on pair-wise energy transfer measurements. A second hierarchical-level of receptor clusters have been described by electron and scanning force microscopies after immuno-gold-labeling of distinct receptor kinds. The origin of these receptor islands at a nanometer scale and island groups at a higher hierarchical (μm) level, has been explained mostly by detergent insoluble glycolipid-enriched complexes known as rafts, or detergent insoluble glycolipids (DIGs). These rafts are the most-likely organizational forces behind at least some kind of receptor clustering [K. Simons et al., Nature 387 (1997) 569]. These models, which have great significance in trans-membrane signaling and intra-membrane and intracellular trafficking, are accentuating the necessity to revisit the Singer-Nicolson fluid mosaic membrane model and substitute the free protein diffusion with a restricted diffusion concept [S.J. Singer et al., Science 175 (1972) 720]. Copyright (C) 1999 Elsevier Science B.V.

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KW - Receptor patterns

KW - Scanning force microscopy

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