Two-dimensional correlation analysis of the reproducibility of high-performance liquid chromatography columns

József Simon, A. Felinger

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Two-dimensional (2D) correlation analysis is a well-established tool in spectroscopy. Despite its versatility in various measurement systems, 2D correlation has not yet become popular in separation science. 2D correlation is seldom used in chromatography; only a few a studies can be found on this topic and most of those publications report about gel chromatography. In the present study, 2D correlation analysis is applied to chromatograms. In this study, a simple method is built for studying the similarities and dissimilarities between a number of chromatograms. We present the applicability of the method by two examples, where the repeatability and reproducibility of the analytical and nonlinear measurements in HPLC are evaluated and demonstrated. In order to validate the results of 2D correlation analysis, they are compared to principal component analysis (PCA). We confirm the equivalence in the interpretation of the results obtained with the two methods of calculation. The results confirm that 2D correlation can be a successful chemometric tool in chromatography.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalJournal of Chromatography A
Volume1384
DOIs
Publication statusPublished - Mar 6 2015

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High performance liquid chromatography
Chromatography
High Pressure Liquid Chromatography
Principal Component Analysis
Principal component analysis
Gel Chromatography
Publications
Spectrum Analysis
Gels
Spectroscopy

Keywords

  • Liquid chromatography
  • Principal component analysis
  • Reproducibility
  • Two-dimensional correlation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Two-dimensional correlation analysis of the reproducibility of high-performance liquid chromatography columns. / Simon, József; Felinger, A.

In: Journal of Chromatography A, Vol. 1384, 06.03.2015, p. 115-123.

Research output: Contribution to journalArticle

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