Tuneable surface modification of PLGA nanoparticles carrying new antitubercular drug candidate

E. Kiss, G. Gyulai, Cs B. Pénzes, M. Idei, K. Horváti, B. Bacsa, Sz. Bősze

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Biodegradable poly(lactic-. co-glycolic acid)(PLGA 50:50) nanoparticles (NPs) were prepared and characterized in terms of size, composition, zeta potential and colloidal stability. Surface modification of PLGA NPs where primary amino groups were introduced to the Pluronic surface layer was developed. This method allows modulation of the charge character of the nanoparticle surface and provides functional groups for chemical reactions useful for targeting while retaining the aggregation stability of the system. The nanoparticles showed significant interaction with model membrane system (DPPC and DPPC. +. DPPG lipid layers) depending on the amount and type of Pluronic applied for stabilization of NPs.A new antitubercular drug candidate was encapsulated into the PLGA NPs. The cellular uptake and the intracellular efficacy against Mycobacterium tuberculosis ( Mtb) of the drug and the drug loaded nanoparticulate systems were investigated. These formulations were successfully taken up by MonoMac6 human monocyte cells and highly enhanced the availability and efficacy of the drug against Mtb which was demonstrated in comparative in vitro experiments.

Original languageEnglish
Pages (from-to)178-186
Number of pages9
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume458
Issue number1
DOIs
Publication statusPublished - Sep 20 2014

Fingerprint

Antitubercular Agents
Surface treatment
drugs
Nanoparticles
nanoparticles
tuberculosis
Poloxamer
Pharmaceutical Preparations
monocytes
Zeta potential
retaining
Lipids
Functional groups
availability
lipids
polylactic acid-polyglycolic acid copolymer
Chemical reactions
chemical reactions
surface layers
Agglomeration

Keywords

  • Antitubercular drug candidate
  • Cellular uptake
  • Membrane affinity of NPs
  • PLGA nanoparticles
  • Pluronic127-amine
  • Surface modification

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

Cite this

Tuneable surface modification of PLGA nanoparticles carrying new antitubercular drug candidate. / Kiss, E.; Gyulai, G.; Pénzes, Cs B.; Idei, M.; Horváti, K.; Bacsa, B.; Bősze, Sz.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 458, No. 1, 20.09.2014, p. 178-186.

Research output: Contribution to journalArticle

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