Tumor necrosis factor-α induces a κB sequence-specific DNA-binding protein in human hepatoblastoma HepG2 cells

R. Banerjee, S. Karpen, M. Siekevitz, G. Lengyel, J. Bauer, G. Acs

Research output: Contribution to journalArticle

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Abstract

Tumor necrosis factor-α is an inducer of acute-phase protein synthesis in liver cells. The mechanism by which tumor necrosis factor-α alters gene expression in these cells is largely unknown. In this study, we demonstrate that tumor necrosis factor-α stimulates human immunodeficiency virus-1 long terminal repeat-promoted gene expression in the human hepatoblastoma HepG2 cell line and increased binding of trans-activating factors to kappa B (κB) DNA sequences. In contrast to lymphocytic cells where the nuclear factors recognizing the κB sequences are activated by both tumor necrosis factor-α and phorbol-12-myristate-13-acetate through a posttranslational mechanism, in HepG2 cells phorbol-12-myristate-13-acetate does not activate these factor(s), and de novo protein synthesis seems to be required in HepG2 cells for gene activation by tumor necrosis factor-α.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalHepatology
Volume10
Issue number6
Publication statusPublished - 1989

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Hepatoblastoma
Hep G2 Cells
DNA-Binding Proteins
Tumor Necrosis Factor-alpha
Acetates
HIV Long Terminal Repeat
Gene Expression
Acute-Phase Proteins
Transcriptional Activation
HIV-1
Cell Line
Liver
Proteins

ASJC Scopus subject areas

  • Hepatology

Cite this

Banerjee, R., Karpen, S., Siekevitz, M., Lengyel, G., Bauer, J., & Acs, G. (1989). Tumor necrosis factor-α induces a κB sequence-specific DNA-binding protein in human hepatoblastoma HepG2 cells. Hepatology, 10(6), 1008-1013.

Tumor necrosis factor-α induces a κB sequence-specific DNA-binding protein in human hepatoblastoma HepG2 cells. / Banerjee, R.; Karpen, S.; Siekevitz, M.; Lengyel, G.; Bauer, J.; Acs, G.

In: Hepatology, Vol. 10, No. 6, 1989, p. 1008-1013.

Research output: Contribution to journalArticle

Banerjee, R, Karpen, S, Siekevitz, M, Lengyel, G, Bauer, J & Acs, G 1989, 'Tumor necrosis factor-α induces a κB sequence-specific DNA-binding protein in human hepatoblastoma HepG2 cells', Hepatology, vol. 10, no. 6, pp. 1008-1013.
Banerjee, R. ; Karpen, S. ; Siekevitz, M. ; Lengyel, G. ; Bauer, J. ; Acs, G. / Tumor necrosis factor-α induces a κB sequence-specific DNA-binding protein in human hepatoblastoma HepG2 cells. In: Hepatology. 1989 ; Vol. 10, No. 6. pp. 1008-1013.
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