Tumor necrosis factor‐α induces a kB sequence‐specific DNA‐binding protein in human hepatoblastoma HepG2 cells

Ranjit Banerjee, Saul Karpen, Miriam Siekevitz, Gabriella Lengyel, Joachim Bauer, George Acs

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tumor necrosis factor‐α is an inducer of acute‐phase protein synthesis in liver cells. The mechanism by which tumor necrosis factor‐α alters gene expression in these cells is largely unknown. In this study, we demonstrate that tumor necrosis factor‐α stimulates human immunodeficiency virus‐1 long terminal repeat‐promoted gene expression in the human hepatoblastoma HepG2 cell line and increased binding of trans‐activating factors to kappa B (kB) DNA sequences. In contrast to lymphocytic cells where the nuclear factors recognizing the kB sequences are activated by both tumor necrosis factor‐α and phorbol‐12‐myristate‐13‐acetate through a posttranslational mechanism, in HepG2 cells phorbol‐12‐myristate‐13‐acetate does not activate these factor(s), and de novo protein synthesis seems to be required in HepG2 cells for gene activation by tumor necrosis factor‐α.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalHepatology
Volume10
Issue number6
DOIs
Publication statusPublished - Dec 1989
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology

Fingerprint Dive into the research topics of 'Tumor necrosis factor‐α induces a kB sequence‐specific DNA‐binding protein in human hepatoblastoma HepG2 cells'. Together they form a unique fingerprint.

  • Cite this