Tumor grade-dependent alterations in the protein kinase C isoform pattern in urinary bladder carcinomas

Attila Varga, Gabriella Czifra, Béla Tállai, Tamás Németh, Ilona Kovács, László Kovács, Tamás Bíró

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51 Citations (Scopus)

Abstract

Objectives: Members of the protein kinase C (PKC) isoenzyme family play central role in the tumorigenesis of several tissues. In this study our goal was to determine the possible alterations in the protein kinase C (PKC) isoform pattern in relation with the different tumor grade in human urinary bladder carcinomas. Methods: Western blot analysis, followed by quantitative densitometry, was performed to define the expression of PKC isoforms in the epithelial tissue of human urinary bladder carcinomas with various tumor grades and in control samples. Results: The human urinary bladder epithelium expressed five PKC isoforms (PKCα, β, δ, ε, ζ), the levels of which differentially altered as a function of tumor grade. Namely, whereas the expressions of PKCβ and δ decreased with increasing grade of the carcinomas, the levels of PKCα, ε, and ζ showed opposite patterns of changes. Conclusions: These grade-dependent alterations in the PKC isoform pattern strongly argue for the central yet antagonistic roles of certain members of the PKC system in malignant transformation of human urinary bladder epithelium.

Original languageEnglish
Pages (from-to)462-465
Number of pages4
JournalEuropean Urology
Volume46
Issue number4
DOIs
Publication statusPublished - Oct 1 2004

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Keywords

  • Isoenzymes
  • Protein kinase C
  • Tumorigenesis
  • Urinary bladder carcinoma

ASJC Scopus subject areas

  • Urology

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