Tumor cells expressing membrane-bound tumor necrosis factor activate macrophages and have a compromised growth in immunosuppressed and immunodeficient mice

Tünde Nagy, Hristos Glavinas, Nóra Szincsák, János Hunyadi, Tamás Jánossy, Ernö Duda, Csaba Vizler, István Juhász

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tumor necrosis factor (TNF)-α producing tumors as vaccines were demonstrated to induce a therapeutic anti-tumor immune response, but their clinical use is limited by the toxicity of soluble TNF. We investigated the growth characteristics and immunomodulatory properties of HeLa cells producing an uncleavable transmembrane form of TNF (preTNF). The growth of the transformed tumors was compromised in both immunosuppressed and severe combined immunodeficient mice; no signs of TNF toxicity were detected. Macrophages co-cultured with the transformed cells showed increased phagocytosis and cytokine production, indicating that activated macrophages may be the mediators of the anti-tumor effect. preTNF producing tumor cells are promising safe anti-tumor vaccine candidates.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalCancer Letters
Volume196
Issue number1
DOIs
Publication statusPublished - Jun 30 2003

    Fingerprint

Keywords

  • Macrophage tumor killing
  • Membrane-bound tumor necrosis factor
  • Severe combined immunodeficient mice
  • Tumor gene therapy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this