Tubulin polymerization-promoting protein (TPPP/p25) is critical for oligodendrocyte differentiation

A. Lehotzky, Pierre Lau, Natália Tokési, Naser Muja, Lynn D. Hudson, J. Ovádi

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

TPPP/p25, a recently identified tubulin polymerization-promoting protein (TPPP), is expressed mainly in myelinating oligodendrocytes of the CNS. Here, we show that TPPP/p25 is strongly upregulated during the differentiation of primary oligodendrocyte cells as well as the CG-4 cell line. The microRNA expression profile of CG-4 cells before and after induction of differentiation was established and revealed differential regulation of a limited subset of micro-RNAs. miR-206, a microRNA predicted to target TPPP/p25, was not detected in oligodendrocytes. Overexpression of miR-206 led to downregulation of TPPP/p25 resulting in inhibition of differentiation. Transfection of siRNAs against TPPP/p25 also inhibited cell differentiation and promoted cell proliferation, providing evidence for an important role of TPPP/p25 during oligodendrogenesis. These results support an essential role for TPPP/p25 in oligodendrocyte differentiation likely via rearrangement of the microtubule system during the process elongation prior to the onset of myelination.

Original languageEnglish
Pages (from-to)157-168
Number of pages12
JournalGLIA
Volume58
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Oligodendroglia
Tubulin
Polymerization
Proteins
MicroRNAs
Microtubules
Transfection
Cell Differentiation
Down-Regulation
Cell Proliferation
Cell Line

Keywords

  • Cytoskeleton
  • MicroRNA
  • Oligodendrocytes
  • TPPP/p25

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Tubulin polymerization-promoting protein (TPPP/p25) is critical for oligodendrocyte differentiation. / Lehotzky, A.; Lau, Pierre; Tokési, Natália; Muja, Naser; Hudson, Lynn D.; Ovádi, J.

In: GLIA, Vol. 58, No. 2, 2010, p. 157-168.

Research output: Contribution to journalArticle

Lehotzky, A. ; Lau, Pierre ; Tokési, Natália ; Muja, Naser ; Hudson, Lynn D. ; Ovádi, J. / Tubulin polymerization-promoting protein (TPPP/p25) is critical for oligodendrocyte differentiation. In: GLIA. 2010 ; Vol. 58, No. 2. pp. 157-168.
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