Tuberoinfundibular peptide of 39 residues is activated during lactation and participates in the suckling-induced prolactin release in rat

Melinda Cservenák, I. Bodnár, Ted B. Usdin, M. Palkóvits, György M. Nagy, A. Dobolyi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Tuberoinfundibular peptide of 39 residues (TIP39) and the PTH-2 receptor (PTH2R) constitute a peptide-receptor neuromodulator system. Based on the abundance of TIP39 fibers and axonal terminals as well as PTH2R-containing neurons and their processes in the hypothalamic para- and periventricular and arcuate nuclei TIP39 has been suggested to play a role in neuroendocrine regulation. We showed previously that TIP39 expression decreased dramatically by adulthood. In the present study, using in situ hybridization histochemistry, real-time RT-PCR, and immunohistochemistry, we found that TIP39 mRNA and peptide expression levels are markedly elevated in the posterior intralaminar complex of the thalamus (PIL) of lactating dams, one of the three locations of TIP39-containing cell bodies in the brain. In addition, in mother rats, these TIP39 neurons showed Fos expression in response to pup exposure. Transection of TIP39 fibers originating in the PIL resulted in an ipsilateral disappearance of TIP39 immunoreactivity throughout the mediobasal hypothalamus of mother rats, suggesting that TIP39 fibers there arise from the PIL. To elucidate the function of TIP39 activation in dams, mothers separated from their pups for 4 h on postpartum d 9 received injection of a PTH2R antagonist into the lateral ventricle 5 min before returning the pups. Blood samples were taken seven times during the experimental period through jugular cannulae. The PTH2R antagonist administered in two different concentrations markedly inhibited suckling-induced elevation of plasma prolactin levels in a dose-dependent manner. These results suggest that TIP39 neurons in the PIL may regulate suckling-induced prolactin release in rat dams.

Original languageEnglish
Pages (from-to)5830-5840
Number of pages11
JournalEndocrinology
Volume151
Issue number12
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Lactation
Prolactin
Receptor, Parathyroid Hormone, Type 2
Neurons
tuberoinfundibular peptide 39
Arcuate Nucleus of Hypothalamus
Peptide Receptors
Paraventricular Hypothalamic Nucleus
Lateral Ventricles
Thalamus
Postpartum Period
Hypothalamus
In Situ Hybridization
Neurotransmitter Agents
Real-Time Polymerase Chain Reaction
Neck
Immunohistochemistry
Messenger RNA
Peptides
Injections

ASJC Scopus subject areas

  • Endocrinology

Cite this

Tuberoinfundibular peptide of 39 residues is activated during lactation and participates in the suckling-induced prolactin release in rat. / Cservenák, Melinda; Bodnár, I.; Usdin, Ted B.; Palkóvits, M.; Nagy, György M.; Dobolyi, A.

In: Endocrinology, Vol. 151, No. 12, 12.2010, p. 5830-5840.

Research output: Contribution to journalArticle

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