Th1/Th17 polarization and acquisition of an arthritogenic phenotype in arthritis-susceptible BALB/c, but not in MHC-matched, arthritis-resistant DBA/2 mice

F. Boldizsár, Oktavia Tarjanyi, P. Németh, Katalin Mikecz, Tibor T. Glant

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Proteoglycan (PG) aggrecan-induced arthritis (PGIA) is a murine model of rheumatoid arthritis (RA). Although BALB/c and DBA/2 mice share the same MHC (H-2d) haplotype, the BALB/c strain is susceptible to PGIA, while DBA/2 mice are resistant. Therefore, these two inbred mouse strains provide an opportunity to study arthritis susceptibility factors excluding the effects of MHC-associated genetic components. The goal of this study was to monitor changes in the cellular composition and activation state following intra-peritoneal (i.p.) immunization to induce PGIA; additionally, we sought to identify new susceptibility factors by comparing PG-induced immune responses in BALB/c and DBA/2 mice. Upon i.p. PG injection, resident naive B1 cells are replaced by both T cells and conventional B cells in the peritoneum of BALB/c mice. These peritoneal T cells produce IFNγ and IL-17, cytokines shown to be important in RA and corresponding arthritis models. Moreover, peritoneal cells can adoptively transfer PGIA to SCID mice, demonstrating their arthritogenic properties. Our results indicate that repeatedly injected antigen leads to the recruitment and activation of immune cells in the peritoneum; these cells then trigger the effector phase of the disease. The migration and activation of Th1/ Th17 cells in the peritoneal cavity in response to PG immunization, which did not occur in the arthritis-resistant DBA/2 strain, may be critical factors of arthritis susceptibility in BALB/c mice.

Original languageEnglish
Pages (from-to)511-522
Number of pages12
JournalInternational Immunology
Volume21
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Inbred DBA Mouse
Arthritis
Phenotype
Aggrecans
Proteoglycans
Peritoneum
Immunization
Rheumatoid Arthritis
T-Lymphocytes
Th17 Cells
Th1 Cells
Inbred Strains Mice
SCID Mice
Interleukin-17
Peritoneal Cavity
Haplotypes
B-Lymphocytes
Cytokines
Antigens
Injections

Keywords

  • Cytokines
  • Peritoneum
  • Rheumatoid arthritis
  • Spleen
  • T cells

ASJC Scopus subject areas

  • Immunology

Cite this

Th1/Th17 polarization and acquisition of an arthritogenic phenotype in arthritis-susceptible BALB/c, but not in MHC-matched, arthritis-resistant DBA/2 mice. / Boldizsár, F.; Tarjanyi, Oktavia; Németh, P.; Mikecz, Katalin; Glant, Tibor T.

In: International Immunology, Vol. 21, No. 5, 2009, p. 511-522.

Research output: Contribution to journalArticle

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