Tritium and iodine labelling of a gastrin like hexapeptide

Marie‐Hélène ‐H Charon, Paul Pham Van Chuong, Botond Penke, Jean‐Louis ‐L Morgat, Marcelle Dubrasquet, Pierre Fromageot

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tyrosine derivatives were introduced onto the N‐terminal deprotected Pentagastrin to yield precursors of labelling. The tritiated derivative Boc‐3,5‐3H2‐Tyr‐Pentagastrin of high specific radioactivity (45 Ci/mmol) was obtained by catalytic dehalogenation of the corresponding di‐iodo‐derivative in the presence of tritium gas. Iodination of Boc‐Tyr‐Pentagastrin by NaI and chloramine T led to the formation of several compounds separated by HPLC. Conditions to obtain the mono‐iodinated non‐oxidized peptide, the only biologically active derivative, were investigated. Analytical chromatographic behaviour as well as biological activity of the tritiated and of the various iodinated peptides were reported.

Original languageEnglish
Pages (from-to)15-36
Number of pages22
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1984

Keywords

  • Gastrin like peptides
  • Iodination
  • Methionine oxidation
  • Reverse‐phase HPLC
  • Tritium and iodine labelling

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry

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  • Cite this

    Charon, MH. H., Van Chuong, P. P., Penke, B., Morgat, JL. L., Dubrasquet, M., & Fromageot, P. (1984). Tritium and iodine labelling of a gastrin like hexapeptide. Journal of Labelled Compounds and Radiopharmaceuticals, 21(1), 15-36. https://doi.org/10.1002/jlcr.2580210103