Trisk 32 regulates IP 3 receptors in rat skeletal myoblasts

Tamás Oláh, János Fodor, Sarah Oddoux, Olga Ruzsnavszky, Isabelle Marty, L. Csernoch

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To date, four isoforms of triadins have been identified in rat skeletal muscle. While the function of the 95-kDa isoform in excitation-contraction coupling has been studied in detail, the role of the 32-kDa isoform (Trisk 32) remains elusive. Here, Trisk 32 overexpression was carried out by stable transfection in L6.G8 myoblasts. Co-localization of Trisk 32 and IP 3 receptors (IP 3R) was demonstrated by immunocytochemistry, and their association was shown by co-immunoprecipitation. Functional effects of Trisk 32 on IP 3-mediated Ca 2+ release were assessed by measuring changes in [Ca 2+] i following the stimulation by bradykinin or vasopressin. The amplitude of the Ca 2+ transients evoked by 20 μM bradykinin was significantly higher in Trisk 32-overexpressing (p∈2+. Similar observations were made when 0.1 μM vasopressin was used to initiate Ca 2+ release. Possible involvement of the ryanodine receptors (RyR) in these processes was excluded, after functional and biochemical experiments. Furthermore, Trisk 32 overexpression had no effect on store-operated Ca 2+ entry, despite a decrease in the expression of STIM1. These results suggest that neither the increased activity of RyR, nor the amplification of SOCE, is responsible for the differences observed in bradykinin- or vasopressin-evoked Ca 2+ transients; rather, they were due to the enhanced activity of IP 3R. Thus, Trisk 32 not only co-localizes with, but directly contributes to, the regulation of Ca 2+ release via IP 3R.

Original languageEnglish
Pages (from-to)599-610
Number of pages12
JournalPflugers Archiv European Journal of Physiology
Volume462
Issue number4
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Skeletal Myoblasts
Bradykinin
Vasopressins
Rats
Protein Isoforms
Ryanodine Receptor Calcium Release Channel
Excitation Contraction Coupling
Myoblasts
Immunoprecipitation
Transfection
Amplification
Muscle
Skeletal Muscle
Immunohistochemistry
Experiments

Keywords

  • Calcium transient
  • Endoplasmic reticulum
  • Inositol 1,4,5-trisphosphate
  • Myoblasts
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Trisk 32 regulates IP 3 receptors in rat skeletal myoblasts. / Oláh, Tamás; Fodor, János; Oddoux, Sarah; Ruzsnavszky, Olga; Marty, Isabelle; Csernoch, L.

In: Pflugers Archiv European Journal of Physiology, Vol. 462, No. 4, 10.2011, p. 599-610.

Research output: Contribution to journalArticle

Oláh, Tamás ; Fodor, János ; Oddoux, Sarah ; Ruzsnavszky, Olga ; Marty, Isabelle ; Csernoch, L. / Trisk 32 regulates IP 3 receptors in rat skeletal myoblasts. In: Pflugers Archiv European Journal of Physiology. 2011 ; Vol. 462, No. 4. pp. 599-610.
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