Trigonella foenum-graecum and trigonella corniculata seed extracts exert a protective action on alcohol toxicity in BRL3A rat liver cells

George Pribac, Aurel Ardelean, Marika Czapar, Eniko Bodo, Liana Mos

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study we have investigated the protective effect of Trigonella seed extract against ethanol induced toxicity in BRL3A rat liver cells. Cells were treated with either 30 mM ethanol alone or together with Trigonella seed extract for 24 h. We have performed various assays in treated cells to evaluate the ability of seeds to prevent the toxic effects of ethanol. Ethanol treatment suppressed the growth of BRL3A rat liver cells and induced cytotoxicity, oxygen radical formation and mitochondrial dysfunction. Incubation of TSE with ethanol increased cell viability in a dose-dependent manner. It caused a decrease in lactate dehydrogenase outflow. Trigonella seed extract reduced the production of TBARS. Apoptosis was observed in ethanol treated cells while TSE reduced apoptosis by decreasing the accumulation of G1 phase cells. The cytoprotective effects of TSE were comparable with those of a positive control. For this we have used silymarin, a known hepatoprotective agent. The data obtained suggest that fenugreek seeds can be considered cytoprotective in ethanol induced liver damage due to their polyphenolic compounds.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalStudia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
Volume19
Issue number1
Publication statusPublished - Dec 1 2009

Keywords

  • Alcohol toxicity
  • BRL3A rat liver cells
  • Trigonella seed extract (TSE)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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