Trichoderma chitinase gene expression confers mould resistance in tobacco

K. Kálai, G. Giczey, A. Mészáros, E. Balázs, F. Dénes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Obtaining resistance against fungal diseases in plants is still a great challenge for the breeders. An alternative strategy is to introduce genes encoding antifungal enzymes into the host plant genome. Trichoderma species are known to have strong antifungal effect partly as a result of their production of extracellular chitinase enzymes, which hydrolyse the main constituent of the fungal cell wall. The 42 kDa endochitinase gene of Trichoderma hamatum was introduced to Nicotiana tabacum plants under the control of the Ca35S promoter and the NOS terminator. The transformed plantlets grown on selective medium showed no morphological or physiological difference as compared to the wild type plants. They all were positive in PCR tests performed to screen a 164 bp sequence of the Trichoderma endochitinase gene. The sequence of the amplified fragment was analysed and showed 100% homology with the Trichoderma hamatum endochitinase gene. We did not detect homology with plant origin chitinase enzymes. Compared to the untransformed control, all transgenic lines showed higher tolerance for Botrytis cinerea in fungal infection experiments. The endochitinase gene could be detected in the first and the second progeny of the transformed lines, too.

Original languageEnglish
Title of host publicationProceedings of the Fifth nternational Symposium on In Vitro Culture and Horticultural Breeding
PublisherInternational Society for Horticultural Science
Pages783-789
Number of pages7
ISBN (Print)906605719X, 9789066057197
DOIs
Publication statusPublished - Nov 2006

Publication series

NameActa Horticulturae
Volume725 II
ISSN (Print)0567-7572

Keywords

  • Botrytis cinerea
  • Fungal resistance
  • Genetic engineering
  • Hydrolytic enzymes
  • Transgenic plant

ASJC Scopus subject areas

  • Horticulture

Fingerprint Dive into the research topics of 'Trichoderma chitinase gene expression confers mould resistance in tobacco'. Together they form a unique fingerprint.

  • Cite this

    Kálai, K., Giczey, G., Mészáros, A., Balázs, E., & Dénes, F. (2006). Trichoderma chitinase gene expression confers mould resistance in tobacco. In Proceedings of the Fifth nternational Symposium on In Vitro Culture and Horticultural Breeding (pp. 783-789). (Acta Horticulturae; Vol. 725 II). International Society for Horticultural Science. https://doi.org/10.17660/actahortic.2006.725.108