Trichloroethanol inhibits ATP-induced membrane currents in cultured HEK 293-hP2X3 cells

L. Köles, Kerstin Wirkner, S. Fürst, Stephan Wnendt, Peter Illes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Membrane currents in response to the application of α,β-methylene ATP (α,β-meATP) were recorded by the whole-cell patch-clamp technique in human embryonic kidney 293 cells transfected with the human P2X3 receptor (HEK 293-hP2X3 cells). Trichloroethanol, the biologically active metabolite of chloral hydrate, but not ethanol itself concentration-dependently and reversibly inhibited the current responses. It was concluded that the reported analgesic effect of chloral hydrate may be due to the interruption of pain transmission in dorsal root ganglia expressing P2X3 receptors. (C) 2000 Elsevier Science B.V.

Original languageEnglish
JournalEuropean Journal of Pharmacology
Volume409
Issue number3
DOIs
Publication statusPublished - Dec 15 2000

Fingerprint

Purinergic P2X3 Receptors
Chloral Hydrate
HEK293 Cells
Cultured Cells
Adenosine Triphosphate
Membranes
Spinal Ganglia
Patch-Clamp Techniques
Analgesics
Ethanol
Kidney
Pain
2,2,2-trichloroethanol

Keywords

  • Ethanol
  • P2X receptor
  • Trichloroethanol

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Trichloroethanol inhibits ATP-induced membrane currents in cultured HEK 293-hP2X3 cells. / Köles, L.; Wirkner, Kerstin; Fürst, S.; Wnendt, Stephan; Illes, Peter.

In: European Journal of Pharmacology, Vol. 409, No. 3, 15.12.2000.

Research output: Contribution to journalArticle

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AU - Fürst, S.

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AU - Illes, Peter

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