Trf4 and Trf5 proteins of Saccharomyces cerevisiae exhibit poly(A) RNA polymerase activity but no DNA polymerase activity

L. Haracska, Robert E. Johnson, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae Trf4 and Trf5 proteins are members of a distinct family of eukaryotic DNA polymerase β-like nucleotidyltransferases, and a template-dependent DNA polymerase activity has been reported for Trf4. To define the nucleotidyltransferase activities associated with Trf4 and Tr5, we purified these proteins from yeast cells and show that whereas both proteins exhibit a robust poly(A) polymerase activity, neither of them shows any evidence of a DNA polymerase activity. The poly(A) polymerase activity, as determined for Trf4, is strictly Mn2+ dependent and highly ATP specific, incorporating AMP onto the free 3′-hydroxyl end of an RNA primer. Unlike the related poly(A) polymerases from other eukaryotes, which are located in the cytoplasm and regulate the stability and translation efficiency of specific mRNAs, the Trf4 and Trf5 proteins are nuclear, and a multiprotein complex associated with Trf4 has been recently shown to polyadenylate a variety of misfolded or inappropriately expressed RNAs which activate their degradation by the exosome. To account for the effects of Trf4/Trf5 proteins on the various aspects of DNA metabolism, including chromosome condensation, DNA replication, and sister chromatid cohesion, we suggest an additional and essential role for the Trf4 and Trf5 protein complexes in generating functional mRNA poly(A) tails in the nucleus.

Original languageEnglish
Pages (from-to)10183-10189
Number of pages7
JournalMolecular and Cellular Biology
Volume25
Issue number22
DOIs
Publication statusPublished - Nov 2005

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Polynucleotide Adenylyltransferase
Saccharomyces cerevisiae Proteins
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Nucleotidyltransferases
Messenger RNA
Proteins
Exosomes
Multiprotein Complexes
Chromatids
Fungal Proteins
Adenosine Monophosphate
Nuclear Proteins
Eukaryota
DNA Replication
Hydroxyl Radical
Saccharomyces cerevisiae
Cytoplasm
Chromosomes
Adenosine Triphosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Trf4 and Trf5 proteins of Saccharomyces cerevisiae exhibit poly(A) RNA polymerase activity but no DNA polymerase activity. / Haracska, L.; Johnson, Robert E.; Prakash, Louise; Prakash, Satya.

In: Molecular and Cellular Biology, Vol. 25, No. 22, 11.2005, p. 10183-10189.

Research output: Contribution to journalArticle

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