Trehalose overproduction affects the stress tolerance of Kluyveromyces marxianus ambiguously

Éva Erdei, M. Molnár, G. Gyémánt, K. Antal, T. Emri, I. Pócsi, János Nagy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A thermotolerant Kluyveromyces marxianus mutant was developed by exposing yeast cultures repeatedly to 48 °C incubation temperature, and the strain was characterized with a significantly increased trehalose content. Unexpectedly, the strain was sensitive to alcohol, osmotic and oxidative stress, which correlated with the increases in the trehalose concentrations. Intracellular glutathione levels declined in both wild-type and mutant cells when exposed to elevating incubation temperatures. Finally, we reached the surprising conclusion that neither trehalose nor glutathione metabolisms should be aimed at in future strain development programs with K. marxianus.

Original languageEnglish
Pages (from-to)7232-7235
Number of pages4
JournalBioresource Technology
Volume102
Issue number14
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Trehalose
tolerance
incubation
Glutathione
yeast
alcohol
Oxidative stress
metabolism
temperature
Metabolism
Yeast
Alcohols
Temperature
mutant
programme
oxidative stress

Keywords

  • Bioethanol
  • Glutathione
  • Thermotolerance
  • Trehalose
  • Yeast

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Trehalose overproduction affects the stress tolerance of Kluyveromyces marxianus ambiguously. / Erdei, Éva; Molnár, M.; Gyémánt, G.; Antal, K.; Emri, T.; Pócsi, I.; Nagy, János.

In: Bioresource Technology, Vol. 102, No. 14, 07.2011, p. 7232-7235.

Research output: Contribution to journalArticle

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