Traumatic axonal injury in the spinal cord evoked by traumatic brain injury

Endre Czeiter, J. Pál, Erzsebet Kovesdi, Peter Bukovics, Janos Luckl, T. Dóczi, A. Büki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Although it is well known that traumatic brain injury (TBI) evokes traumatic axonal injury (TAI) within the brain, TBI-induced axonal damage in the spinal cord (SC) has been less extensively investigated. Detection of such axonal injury in the spinal cord would further the complexity of TBI while also challenging some functional neurobehavioral endpoints frequently used to assess recovery in various models of TBI. To assess TAI in the spinal cord associated with TBI, we analyzed the craniocervical junction (CCJ), cervico-thoracic (CT), and thoraco-lumber (ThL) spinal cord in a rodent model of impact acceleration of TBI of varying severities. Rats were transcardially fixed with aldehydes at 2, 6, and 24 h post-injury (n = 36); each group included on sham-injured rodent. Semi-serial vibratome sections were reacted with antibodies targeting TAI via alteration in cytoskeletal integrity or impaired axonal transport. Consistent with previous observations in this model, the CCJ contained numerous injured axons. Immunoreactive, damaged axonal profiles were also detected as caudal, as the ThL spinal cord displayed morphological characteristics entirely consistent with those described in the brainstem and the CCJ. Quantitative analyses demonstrated that the occurrence and extent of TAI is positively associated with the impact/energy of injury and negatively with the distance from the brainstem. These observations show that TBI can evoke TAI in regions remote from the injury site, including the spinal cord itself. This finding is relevant to shaken baby syndrome as well as during the analysis of data in functional recovery in various models of TBI.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalJournal of Neurotrauma
Volume25
Issue number3
DOIs
Publication statusPublished - Mar 1 2008

Fingerprint

Spinal Cord Injuries
Spinal Cord
Wounds and Injuries
Brain Stem
Rodentia
Shaken Baby Syndrome
Axonal Transport
Traumatic Brain Injury
Aldehydes
Brain Injuries
Axons
Thorax
Antibodies

Keywords

  • Animal studies
  • Axonal injury
  • Models of injury
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Traumatic axonal injury in the spinal cord evoked by traumatic brain injury. / Czeiter, Endre; Pál, J.; Kovesdi, Erzsebet; Bukovics, Peter; Luckl, Janos; Dóczi, T.; Büki, A.

In: Journal of Neurotrauma, Vol. 25, No. 3, 01.03.2008, p. 205-213.

Research output: Contribution to journalArticle

Czeiter, Endre ; Pál, J. ; Kovesdi, Erzsebet ; Bukovics, Peter ; Luckl, Janos ; Dóczi, T. ; Büki, A. / Traumatic axonal injury in the spinal cord evoked by traumatic brain injury. In: Journal of Neurotrauma. 2008 ; Vol. 25, No. 3. pp. 205-213.
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