Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish

Mónika Szabó, Ferenc Müller, János Kiss, Carolin Balduf, Uwe Strähle, Ferenc Olasz

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage λ causes transposition primarily into the vicinity of the λ operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.

Original languageEnglish
Pages (from-to)46-50
Number of pages5
JournalFEBS letters
Volume550
Issue number1-3
DOIs
Publication statusPublished - Aug 28 2003

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Keywords

  • Gli1
  • IS30 transposase
  • Protein fusion
  • Site-directed integration
  • Zebrafish
  • λ cI repressor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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