Transport properties of the multidrug resistance-associated protein (MRP) in human tumour cells

Research output: Contribution to journalArticle

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Abstract

In this paper we demonstrate that the expression of the multidrug resistance-associated protein (MRP) in a variety of intact human tumour cells results in the ATP-dependent, mutually exclusive extrusion of both the acetoxymethyl ester and the free anion forms of the fluorescent dye calcein, as well as that of a fluorescent pyrenemaleimide-glutathione conjugate. The MRP-dependent transport of all these three model compounds closely correlates with the expression level of MRP and is cross-inhibited by hydrophobic anticancer drugs, by reversing agents for MDR1, and also by compounds not influencing MDR1, such as hydrophobic anions, alkylating agents, and inhibitors of organic anion transporters. Cellular glutathione depletion affects neither the MRP-dependent extrusion of calcein AM or free calcein, nor its modulation by most hydrophobic or anionic compounds, although eliminating the cross-inhibitory effect of glutathione conjugates. These results suggest that the outward pumping of both hydrophobic uncharged and water-soluble anionic compounds, including glutathione conjugates, is an inherent property of MRP, and offer sensitive methods for the functional diagnostics of this transport protein as well as for the rapid screening of drug-resistance modulating agents.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalFEBS Letters
Volume383
Issue number1-2
DOIs
Publication statusPublished - Mar 25 1996

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Multidrug Resistance-Associated Proteins
Transport properties
Tumors
Cells
Glutathione
Neoplasms
Anions
Extrusion
Organic Anion Transporters
Alkylating Agents
Protein Transport
Fluorescent Dyes
Drug Resistance
Pharmaceutical Preparations
Carrier Proteins
Screening
Esters
Adenosine Triphosphate
Modulation
Water

Keywords

  • Calcein
  • Drug resistance
  • Fluorometry
  • Immunoblotting
  • Multidrug resistance-associated protein
  • Multidrug-resistance protein
  • P-glycoprotein
  • Transport assay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Transport properties of the multidrug resistance-associated protein (MRP) in human tumour cells. / Holló, Zsolt; Homolya, L.; Hegedűs, T.; Sarkadi, B.

In: FEBS Letters, Vol. 383, No. 1-2, 25.03.1996, p. 99-104.

Research output: Contribution to journalArticle

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