The transport of acetylcholinesterase (AChE) and choline acetyltransferase (ChAc) were investigated by biochemical and histochemical methods. After ligature of one of the sciatic nerves of the rat for varying times 4, 14, 20 and 44 h the normal levels and the accumulation of AChE and ChAc activities were investigated. The results infer that there is a rapid accumulation of AChE activity just proximal to the ligature, while the increase in ChAc activity is less pronounced. Distal to the ligature the level of AChE is above the control value and in contrast, the ChAc activity is significantly decreased. Histochemical demonstration of the two enzymes indicated that they were in the cholinergic axons. The reaction end product produced by AChE occurred within vesicles and neurotubules, while the end product due to ChAc appeared to be free in the axoplasm, bound to neurofilaments and on the outer surface of vesicles and tubules.
|Number of pages||6|
|Journal||Journal of Neurochemistry|
|Publication status||Published - 1973|
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience