Transmission of Aujeszky's disease (Pseudorabies) virus is blocked by trypsin treatment of transferred embryos

I. Medveczky, L. Solti, J. Haraszti, G. Ronay, K. Ekes, S. Belak, E. Tury, J. Seregi, J. Varga

Research output: Contribution to journalArticle

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Abstract

Four seronegative sows from a farm free of Aujeszky's disease (AD) were artificially inseminated and concurrently infected intranasally and intrauterinally with 105 dose of a virulent strain of Aujeszky's disease virus (ADV). The embryos were recovered surgically 5 d later. The virus could be re-isolated from nasal and vaginal swabs and from different parts of the genital organs (ovary, oviduct, uterus and cervix) of all infected sows. In 2 of the infected sows ADV was also found in the flushing medium used for embryo recovery. The results of virus isolation were confirmed by dot-blot hybridization and histological findings. Embryos with an intact zona pellucida (ZP) collected from the 4 donors were treated twice with 0.25% trypsin solution for 1 min each time prior to transfer into a seronegative sow. The recipient sow and her offspring remained ADV-seronegative on Day 28 after farrowing, indicating that the transmission of ADV had been blocked during the embryo transfer. Untreated control embryos transferred into AD- free recipients caused seroconversion based on the serum neutralization test carried out 40 d later.

Original languageEnglish
Pages (from-to)1357-1365
Number of pages9
JournalTheriogenology
Volume46
Issue number8
DOIs
Publication statusPublished - Dec 1996

Fingerprint

Suid Herpesvirus 1
Aujeszky disease
Suid herpesvirus 1
Trypsin
trypsin
sows
embryo (animal)
Embryonic Structures
Pseudorabies
Viruses
Neutralization Tests
Zona Pellucida
Genitalia
Oviducts
Embryo Transfer
Therapeutics
viruses
Nose
Cervix Uteri
zona pellucida

Keywords

  • Aujeszky's disease
  • Embryo transfer
  • Pseudorabies
  • SHV-1
  • Trypsin treatment

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Transmission of Aujeszky's disease (Pseudorabies) virus is blocked by trypsin treatment of transferred embryos. / Medveczky, I.; Solti, L.; Haraszti, J.; Ronay, G.; Ekes, K.; Belak, S.; Tury, E.; Seregi, J.; Varga, J.

In: Theriogenology, Vol. 46, No. 8, 12.1996, p. 1357-1365.

Research output: Contribution to journalArticle

Medveczky, I. ; Solti, L. ; Haraszti, J. ; Ronay, G. ; Ekes, K. ; Belak, S. ; Tury, E. ; Seregi, J. ; Varga, J. / Transmission of Aujeszky's disease (Pseudorabies) virus is blocked by trypsin treatment of transferred embryos. In: Theriogenology. 1996 ; Vol. 46, No. 8. pp. 1357-1365.
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