Translocon pores in the endoplasmic reticulum are permeable to small anions

Beáta Lizák, Ibolya Czegle, Miklós Csala, Angelo Benedetti, József Mandl, Gábor Bánhegyi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Contribution of translocon peptide channels to the permeation of low molecular mass anions was investigated in rat liver microsomes. Puromycin, which purges translocon pores of nascent polypeptides, creating additional empty pores, raised the microsomal uptake of radiolabeled UDP-glucuronic acid, while it did not increase the uptake of glucose-6-phosphate or glutathione. The role of translocon pores in the transport of small anions was also investigated by measuring the effect of puromycin on the activity of microsomal enzymes with intraluminal active sites. The mannose-6-phosphatase activity of glucose-6-phosphatase and the activity of UDP-glucuronosyltransferase were elevated upon addition of puromycin, but glucose-6-phosphatase and β-glucuronidase activities were not changed. The increase in enzyme activities was due to a better access of the substrates to the luminal compartment rather than to activation of the enzymes. Antibody against Sec61 translocon component decreased the activity of UDP-glucuronosyltransferase and antagonized the effect of puromycin. Similarly, the addition of the puromycin antagonist anisomycin or treatments of microsomes, resulting in the release of attached ribosomes, prevented the puromycindependent increase in the activity. Mannose-6-phosphatase and UDP-glucuronosyltransferase activities of smooth microsomal vesicles showed higher basal latencies that were not affected by puromycin. In conclusion, translationally inactive, ribosome-bound translocons allow small anions to cross the endoplasmic reticulum membrane. This pathway can contribute to the nonspecific substrate supply of enzymes with intraluminal active centers.

Original languageEnglish
Pages (from-to)C511-C517
JournalAmerican Journal of Physiology - Cell Physiology
Volume291
Issue number3
DOIs
Publication statusPublished - Sep 13 2006

Keywords

  • Glucose-6-phosphatase
  • Puromycin
  • UDP-glucuronosyltransferase
  • β-glucuronidase

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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