Translocation of pp60c-src to the cytoskeleton during platelet aggregation

A. R. Horvath, L. Muszbek, S. Kellie

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The high amount of pp60c-src in platelets has led to speculation that this kinase is responsible for tyrosine-specific phosphorylation of cellular proteins during platelet activation by different agonists, and is, therefore, implicated in signal transduction of these cells. Unlike pp60v-src, the association of which with the cytoskeleton appears to be a prerequisite for transformation, pp60c-src is detergent-soluble in fibroblasts overexpressing the c-src gene, and its role in normal cellular function remains elusive. To gain a better understanding of the function of pp60c-src we have investigated the subcellular distribution of pp60c-src and its relationship to the cytoskeleton during platelet activation. Quantitative immunoblotting and immunoprecipitation have revealed that pp60c-src is detergent-soluble in resting platelets, while 40% of total platelet pp60c-src becomes associated with the cytoskeletal fraction upon platelet activation. We have also shown that a small pool of pp60c-src is associated with the membrane skeletal fraction which remains unchanged during the activation process. The interaction of pp60c-src with cytoskeletal proteins strongly correlates with aggregation and is mediated by GPIIb/IIIa receptor-fibrinogen binding. We suggest that the translocation of pp60c-src to the cytoskeleton and its association with cytoskeletal proteins may regulate tyrosine phosphorylation in platelets.

Original languageEnglish
Pages (from-to)855-861
Number of pages7
JournalEMBO Journal
Volume11
Issue number3
Publication statusPublished - 1992

Fingerprint

Platelets
Cytoskeleton
Platelet Aggregation
Platelet Activation
Blood Platelets
Agglomeration
Cytoskeletal Proteins
Detergents
Tyrosine
Chemical activation
Phosphorylation
src Genes
Platelet Glycoprotein GPIIb-IIIa Complex
Immunoprecipitation
Immunoblotting
Fibrinogen
Signal Transduction
Association reactions
Phosphotransferases
Fibroblasts

Keywords

  • Cytoskeleton
  • GPIIbIIIa
  • Phosphorylation
  • Tyrosine kinase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Translocation of pp60c-src to the cytoskeleton during platelet aggregation. / Horvath, A. R.; Muszbek, L.; Kellie, S.

In: EMBO Journal, Vol. 11, No. 3, 1992, p. 855-861.

Research output: Contribution to journalArticle

Horvath, A. R. ; Muszbek, L. ; Kellie, S. / Translocation of pp60c-src to the cytoskeleton during platelet aggregation. In: EMBO Journal. 1992 ; Vol. 11, No. 3. pp. 855-861.
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