Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells

Attila Gábor Szöllosi, Attila Oláh, István Balázs Tóth, Ferenc Papp, Gabriella Czifra, György Panyi, Tamás Bíró

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20 Citations (Scopus)

Abstract

Our goal was to investigate the effect of heat shock on human monocyte-derived dendritic cells (DCs) and to dissect the role of thermosensitive transient receptor potential (TRP) channels in the process. We provide evidence that a short heat shock challenge (43 C) decreased the endocytotic activity of the DCs and that this effect could be alleviated by the RNAi-mediated knockdown of TRPV2 but, importantly, not by the pharmacological (antagonists) or molecular (RNAi) suppression of TRPV1 and TRPV4 activities/levels. Likewise, the heat shock-induced robust membrane currents were selectively and markedly inhibited by TRPV2 "silencing" whereas modulation of TRPV1 and TRPV4 activities, again, had no effect. These intriguing data introduce TRPV2-coupled signaling as a key player in mediating the cellular actions of heat shock on DCs.

Original languageEnglish
Pages (from-to)1440-1445
Number of pages6
JournalFEBS letters
Volume587
Issue number9
DOIs
Publication statusPublished - May 2 2013

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Keywords

  • Human dendritic cell
  • Phagocytosis
  • RNA interference
  • TRPV2
  • Thermosensitive transient receptor
  • potential (TRP) ion channel

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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