Transient increase of raf protein kinase-like immunoreactivity in the rat dentate gyrus during long-term potentiation

A. Mihály, Zoltán Oláh, Manfred Krug, Ulrich Kuhnt, Hansjürgen Matthies, Ulf R. Rapp, Ferenc Joó

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Altered levels of cellular raf proteins (products of the raf protooncogenes) have been shown in the neurons of the dentate fascia of rats in response to high-frequency stimulation, with light microscopic immunohistochemistry by using polyclonal antibodies. No raf-1-like staining was seen in unstimulated tissue, while the pan-raf antibodies revealed immunoreactivity in the cytoplasm of neurons in the Ammon's horn and dentate fascia of rats and guinea pigs. The induction of long-term potentiation in the dentate fascia of freely-moving rats triggered the appearance of raf-1-like staining and increased the number of granule cells with pan-raf-like immunoreactivity. Since these proteins are serine/threonine-specific protein kinases, their appearance in long-term potentiation may indicate the activation of important cell membrane - nucleus transduction pathways.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalNeuroscience Letters
Volume116
Issue number1-2
DOIs
Publication statusPublished - Aug 14 1990

Fingerprint

raf Kinases
Long-Term Potentiation
Dentate Gyrus
Protein Kinases
Staining and Labeling
Neurons
Antibodies
Protein-Serine-Threonine Kinases
Cell Nucleus
Hippocampus
Guinea Pigs
Cytoplasm
Proteins
Cell Count
Immunohistochemistry
Cell Membrane
Light

Keywords

  • Dentate fascia
  • Immunohistochemistry
  • Long-term potentiation
  • Protein kinase
  • Protooncogene

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transient increase of raf protein kinase-like immunoreactivity in the rat dentate gyrus during long-term potentiation. / Mihály, A.; Oláh, Zoltán; Krug, Manfred; Kuhnt, Ulrich; Matthies, Hansjürgen; Rapp, Ulf R.; Joó, Ferenc.

In: Neuroscience Letters, Vol. 116, No. 1-2, 14.08.1990, p. 45-50.

Research output: Contribution to journalArticle

Mihály, A. ; Oláh, Zoltán ; Krug, Manfred ; Kuhnt, Ulrich ; Matthies, Hansjürgen ; Rapp, Ulf R. ; Joó, Ferenc. / Transient increase of raf protein kinase-like immunoreactivity in the rat dentate gyrus during long-term potentiation. In: Neuroscience Letters. 1990 ; Vol. 116, No. 1-2. pp. 45-50.
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