Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior

Beatrice Viale, Lin Song, Volodymyr Petrenko, Anne Laure Wenger Combremont, Alessandro Contestabile, Riccardo Bocchi, Patrick Salmon, Alan Carleton, Lijia An, Laszlo Vutskits, J. Kiss

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

During development, the precise implementation of molecular programs is a key determinant of proper dendritic development. Here, we demonstrate that canonical Wnt signaling is active in dendritic bundle-forming layer II pyramidal neurons of the rat retrosplenial cortex during dendritic branching and spine formation. Transient downregulation of canonical Wnt transcriptional activity during the early postnatal period irreversibly reduces dendritic arbor architecture, leading to long-lasting deficits in spatial exploration and/or navigation and spatial memory in the adult. During the late phase of dendritogenesis, canonical Wnt-dependent transcription regulates spine formation and maturation. We identify neurotrophin-3 as canonical Wnt target gene in regulating dendritogenesis. Our findings demonstrate how temporary imbalance in canonical Wnt signaling during specific time windows can result in irreversible dendritic defects, leading to abnormal behavior in the adult. Viale et al. demonstrate that transient downregulation of canonical Wnt signaling transcriptional activity during early postnatal period leads to irreversible decrease in dendritic complexity and spine number. These long-term defects result in abnormal behavior in the adult rats. In the late phase, reduction in Wnt signaling affects only spine number.

Original languageEnglish
Pages (from-to)1487-1502.e6
JournalCell Reports
Volume27
Issue number5
DOIs
Publication statusPublished - Apr 30 2019

Fingerprint

Dendritic Spines
Deregulation
Pyramidal Cells
Neurons
Spine
Down-Regulation
Neurotrophin 3
Defects
Rats
Transcription
Genes
Navigation
Data storage equipment
Spatial Memory

Keywords

  • dendrite
  • dendritogenesis
  • retrosplenial
  • spatial navigation
  • spine
  • TCF4
  • Wnt
  • β-catenin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior. / Viale, Beatrice; Song, Lin; Petrenko, Volodymyr; Wenger Combremont, Anne Laure; Contestabile, Alessandro; Bocchi, Riccardo; Salmon, Patrick; Carleton, Alan; An, Lijia; Vutskits, Laszlo; Kiss, J.

In: Cell Reports, Vol. 27, No. 5, 30.04.2019, p. 1487-1502.e6.

Research output: Contribution to journalArticle

Viale, B, Song, L, Petrenko, V, Wenger Combremont, AL, Contestabile, A, Bocchi, R, Salmon, P, Carleton, A, An, L, Vutskits, L & Kiss, J 2019, 'Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior', Cell Reports, vol. 27, no. 5, pp. 1487-1502.e6. https://doi.org/10.1016/j.celrep.2019.04.026
Viale, Beatrice ; Song, Lin ; Petrenko, Volodymyr ; Wenger Combremont, Anne Laure ; Contestabile, Alessandro ; Bocchi, Riccardo ; Salmon, Patrick ; Carleton, Alan ; An, Lijia ; Vutskits, Laszlo ; Kiss, J. / Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior. In: Cell Reports. 2019 ; Vol. 27, No. 5. pp. 1487-1502.e6.
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