During the photocycle of bacteriorhodopsin in the purple membrane of Halobacteria halobium protons are pumped through the membrane. In purple membrane suspension the release and uptake of protons cause a transient electric conductivity change. This change is measured in AC electric field oriented purple membrane suspension. The signal is strongly and nonlinearly orientational dependent. The time course of the electric signal is analyzed and it is shown that proton release coincides with the decay of the M-state and the proton uptake with the decay of the O-state.
ASJC Scopus subject areas
- Physics and Astronomy(all)