Transglutaminase 2 is expressed and active on the surface of human monocyte-derived dendritic cells and macrophages

Judit Hodrea, Máté Á Demény, Gyöngyike Majai, Zsolt Sarang, Ilma Rita Korponay-Szabó, László Fésüs

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The multifunctional enzyme, transglutaminase 2 (TG2), can be found intracellularly, in the extracellular matrix and on the cell surface. Cell surface TG2 (csTG2) could not be detected by TG2-specific antibodies or autoantibodies on immunocompetent cells. A supposedly csTG2-specific antibody, 6B9, was recently shown to actually react with CD44. Though the importance of TG2-mediated deamidation of gluten in the pathogenesis of celiac disease has been well recognized, it is not known in which intestinal cells or cell compartment the deamidation occurs. Duodenal dendritic cells (DCs) can be directly involved in gluten-reactive T-cell activation. Here we use blood monocyte-derived dendritic cells (iDC) and macrophages (MΦ) as a model for intestinal antigen-presenting cells (APCs) and show that they contain large amounts of TG2. We found that TG100, a commercial TG2-specific monoclonal antibody can recognize TG2 on the surface of these cells, that is monocyte-derived APCs express surface-associated TG2. TG2 expression was found on the surface of individual tunica propria cells in frozen small bowel tissue sections from both normal and celiac subjects. We also demonstrate that the pool of TG2 on the surface of iDCs can be catalytically active, hence it might directly be involved in the deamidation of gliadin peptides. Bacterial lipopolysaccharide (LPS) increased the level of TG2 on the surface of maturing DCs, supporting the hypothesis that an unspecific inflammatory process in the gut may expose more transglutaminase activity.

Original languageEnglish
Pages (from-to)74-81
Number of pages8
JournalImmunology Letters
Volume130
Issue number1-2
DOIs
Publication statusPublished - May 2010

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Dendritic Cells
Monocytes
Macrophages
Glutens
Antigen-Presenting Cells
transglutaminase 2
Multifunctional Enzymes
Gliadin
Transglutaminases
Antibodies
Celiac Disease
Abdomen
Autoantibodies
Extracellular Matrix
Lipopolysaccharides
Monoclonal Antibodies
T-Lymphocytes
Peptides

Keywords

  • Celiac disease
  • Dendritic cells
  • Macrophages
  • Transglutaminase 2 (TG2)

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Transglutaminase 2 is expressed and active on the surface of human monocyte-derived dendritic cells and macrophages. / Hodrea, Judit; Demény, Máté Á; Majai, Gyöngyike; Sarang, Zsolt; Korponay-Szabó, Ilma Rita; Fésüs, László.

In: Immunology Letters, Vol. 130, No. 1-2, 05.2010, p. 74-81.

Research output: Contribution to journalArticle

Hodrea, Judit ; Demény, Máté Á ; Majai, Gyöngyike ; Sarang, Zsolt ; Korponay-Szabó, Ilma Rita ; Fésüs, László. / Transglutaminase 2 is expressed and active on the surface of human monocyte-derived dendritic cells and macrophages. In: Immunology Letters. 2010 ; Vol. 130, No. 1-2. pp. 74-81.
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