Transgenic expression of bovine neonatal Fc receptor in mice boosts immune response and improves hybridoma production efficiency without any sign of autoimmunity

Zita Schneider, Judit Cervenak, Mária Baranyi, Krisztián Papp, J. Prechl, Glória László, A. Erdei, I. Kacskovics

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The overexpression of the bovine neonatal Fc receptor (bFcRn) in transgenic (Tg) mice boosts humoral immune response with increased numbers of antigen-specific spleen cells and a potent humoral immune response against weakly immunogenic targets. One of the interesting questions surrounding this enhanced immune response is whether these Tg mice generate higher number of antigen-specific hybridomas. To address this question, we immunized these Tg mice and wild type (wt) controls with trinitrophenylated proteins, generated hybridomas and analyzed their numbers and specificities. We observed that Tg mice generated a 3-5 fold increase in antigen-specific IgG titers and had significantly larger spleens containing higher number of antigen-specific B cells and plasma cells, analyzed by ELISA and ELISPOT assays. Fusion of the isolated splenocytes with standard mouse myeloma cells (SP2/0-Ag14) resulted in a 2-4 fold elevation of hybridization frequency for the hapten, or carrier-specific IgG positive microcultures, in Tg mice compared to controls. In addition, as augmented immune reactivity leads to autoimmunity in some genetically modified mouse strains, we analyzed autoreactive antibody levels in serum samples derived from elderly bFcRn Tg mice by a protein chip assay. In contrast to the sample from the MRL/lpr mouse suffering from autoimmunity, we did not detect autoantibodies in bFcRn Tg mice or the wt controls. Based on these and our earlier data, we propose that Tg mice that overexpress bFcRn offer major advantages in monoclonal Ab production.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalImmunology Letters
Volume137
Issue number1-2
DOIs
Publication statusPublished - Jun 30 2011

Fingerprint

Hybridomas
Autoimmunity
Transgenic Mice
Antigens
Humoral Immunity
Spleen
Immunoglobulin G
Inbred MRL lpr Mouse
Enzyme-Linked Immunospot Assay
Protein Array Analysis
neonatal Fc receptors
Haptens
Plasma Cells
Autoantibodies
B-Lymphocytes
Enzyme-Linked Immunosorbent Assay
Antibodies
Serum

Keywords

  • Autoimmunity
  • Hybridoma
  • Monoclonal antibody
  • Neonatal Fc receptor (FcRn)
  • Transgenic mouse

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Transgenic expression of bovine neonatal Fc receptor in mice boosts immune response and improves hybridoma production efficiency without any sign of autoimmunity. / Schneider, Zita; Cervenak, Judit; Baranyi, Mária; Papp, Krisztián; Prechl, J.; László, Glória; Erdei, A.; Kacskovics, I.

In: Immunology Letters, Vol. 137, No. 1-2, 30.06.2011, p. 62-69.

Research output: Contribution to journalArticle

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