Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality

Cecília Tamás, Boglárka N. Kisgyörgy, M. Rakszegi, Mark D. Wilkinson, Moon Sik Yang, L. Láng, L. Tamás, Z. Bedő

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

An amaranth (Amaranthus hypochondriacus) albumin gene, encoding the 35-kDa AmA1 protein of the seed, with a high content of essential amino acids, was used in the biolistic transformation of bread wheat (Triticum aestivum L.) variety Cadenza. The transformation cassette carried the ama1 gene under the control of a powerful wheat endosperm-specific promoter (1Bx17 HMW-GS). Southern-blot analysis of T1 lines confirmed the integration of the foreign gene, while RT-PCR and Western-blot analyses of the samples confirmed the transcription and translation of the transgene. The effects of the extra albumin protein on the properties of flour, produced from bulked T2 seeds, were calculated using total protein and essential amino acid content analysis, polymeric/monomeric protein and HMW/LMW glutenin subunit ratio measurements. The results indicated that not only can essential amino acid content be increased, but some parameters associated with functional quality may also be improved because of the expression of the AmA1 protein.

Original languageEnglish
Pages (from-to)1085-1094
Number of pages10
JournalPlant Cell Reports
Volume28
Issue number7
DOIs
Publication statusPublished - Jun 2009

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Triticum aestivum
nutritive value
essential amino acids
genetically modified organisms
wheat
albumins
proteins
Amaranthus hypochondriacus
biolistics
genes
glutenins
seeds
Southern blotting
translation (genetics)
endosperm
transgenes
flour
Western blotting
transcription (genetics)
protein synthesis

Keywords

  • Amaranth albumin
  • Essential amino acid
  • Functional quality
  • Nutritional quality
  • Transformation
  • Wheat

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality. / Tamás, Cecília; Kisgyörgy, Boglárka N.; Rakszegi, M.; Wilkinson, Mark D.; Yang, Moon Sik; Láng, L.; Tamás, L.; Bedő, Z.

In: Plant Cell Reports, Vol. 28, No. 7, 06.2009, p. 1085-1094.

Research output: Contribution to journalArticle

Tamás, Cecília ; Kisgyörgy, Boglárka N. ; Rakszegi, M. ; Wilkinson, Mark D. ; Yang, Moon Sik ; Láng, L. ; Tamás, L. ; Bedő, Z. / Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality. In: Plant Cell Reports. 2009 ; Vol. 28, No. 7. pp. 1085-1094.
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